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pMKO.1-shSpc25

Plasmid

#160960

Purpose

Spc25 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Publication

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Spc25 shRNA (Spc25 Mouse)

Use

RNAi and Retroviral

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4134029

Plasmid

#152904

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134394

Plasmid

#152845

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;TapTag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133858

Plasmid

#152841

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133135

Plasmid

#152735

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;CBP

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133865

Plasmid

#152674

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133708

Plasmid

#152679

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133787

Plasmid

#152569

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;TRX

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4134187

Plasmid

#152565

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134248

Plasmid

#152513

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133805

Plasmid

#152483

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;CBP

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4134387

Plasmid

#152445

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;6xHis-003

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4133375

Plasmid

#152398

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;S-tag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046638

Plasmid

#153941

Purpose

Yeast expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046622

Plasmid

#153904

Purpose

Yeast expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

June 12, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134365

Plasmid

#152044

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;TapTag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pSADuet148

Plasmid

#121910

Purpose

Charge-introduced NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Publication

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1385 TDP43 RRM-GFP VLIM-S

Plasmid

#118792

Purpose

To test the effect of sequence on TDP43 RRM-GFP droplet properties

Depositor

Rajat Rohatgi

Publication

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All VLIM to S in CTD of TDP43 RRM-GFP reporter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1384 TDP43 RRM-GFP FYW-L

Plasmid

#118793

Purpose

To test the effect of sequence on TDP43 RRM-GFP droplet properties

Depositor

Rajat Rohatgi

Publication

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Expression

Mammalian

Mutation

All FYW to L in CTD of TDP43 RRM-GFP reporter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pOZ-FH-C Bach1 K52R

Plasmid

#73645

Purpose

Retroviral expression of Bach1 K52Rmutant (helicase null)

Depositor

Sharon Cantor

Insert

Bach1 (BACH1 Human)

Use

Retroviral

Tags

FLAG and His

Expression

Mammalian

Mutation

K52R (helicase null)

Promoter

unknown

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only