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Unc5c.y-AP-His

Plasmid

#72055

Purpose

Expresses the extracellular region of the Unc5C, isoform y protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.y (Unc5c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5d.a-AP-His

Plasmid

#72057

Purpose

Expresses the extracellular region of the Unc5D, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5d.a (Unc5d Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5a.z-AP-His

Plasmid

#72051

Purpose

Expresses the extracellular region of the Unc5A, isoform z protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5a.z (Unc5a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5a.a-Fc-His

Plasmid

#72176

Purpose

Expresses the extracellular region of the Unc5A, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5a.a (Unc5a Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5d.a-Fc-His

Plasmid

#72183

Purpose

Expresses the extracellular region of the Unc5D, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5d.a (Unc5d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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RCAS (B) flag Lunatic Fringe (CT#471)

Plasmid

#13980

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Lunatic Fringe (LFNG Chicken)

Use

Retroviral; Avian expression

Tags

Flag

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 BMPR2

Plasmid

#67127

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BMPR2 (BMPR2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pRVH1-hygro shStx3

Plasmid

#40071

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Stx3 shRNA-1

Use

Retroviral

Promoter

H1

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
M26L DJ-1 TEV site pET15b

Plasmid

#60686

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

Insert

M26L DJ-1 (PARK7 Human)

Tags

6xHis

Expression

Bacterial

Mutation

M26L and TEV site was inserted (see comments belo…

Promoter

T7

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
Neo1.c-AP-His

Plasmid

#71965

Purpose

Expresses the extracellular region of the Neogenin 1, isoform c protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Neo1.c (Neo1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Unc5c.z-Fc-His

Plasmid

#72182

Purpose

Expresses the extracellular region of the Unc5C, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5c.z (Unc5c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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RCAS (A) flag Lunatic Fringe (CT#479)

Plasmid

#13979

Depositor

Cliff Tabin

Article

Laufer et al Nature. 1997 Mar 27. 386(6623):366-73.

Insert

Lunatic Fringe (LFNG Chicken)

Use

Retroviral; Avian expression

Tags

Flag

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
Unc5d.z-Fc-His

Plasmid

#72185

Purpose

Expresses the extracellular region of the Unc5D, isoform z protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Unc5d.z (Unc5d Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSG5-H-Ras_V12-S35

Plasmid

#39511

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 S35 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and T35S

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-G37

Plasmid

#39512

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 G37 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and E37G

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-H-Ras_V12-C40

Plasmid

#39515

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 C40 (HRAS Human)

Use

Eukaryotic

Mutation

G12V and Y40C

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pUC19_CoV-2_frD2_ΔE67 3*

Plasmid

#253186

Purpose

For recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

CoV-2_frD2_ΔE67 ORF3*

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD_S Omicron BA.1

Plasmid

#253116

Purpose

For recombinant virus rescue; encodes fragment D of SARS-CoV-2 (nt20950-29867), Wuhan backbone with Omicron BA.1 spike; chimeric

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron BA.1 spike

Expression

Bacterial

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_CoV-2_frD1_XBB.1.5 S

Plasmid

#253119

Purpose

For recombinant virus rescue; encodes fragment D1 of SARS-CoV-2 (nt20950-25522), Omicron isolate XBB.1.5 Spike

Depositor

Thomas Klimkait

Article

Kipfer et al Elife. 2023 Nov 21;12:RP89035. doi: 10.7

Insert

SARS-CoV-2 Omicron XBB.1.5 spike

Expression

Bacterial

Mutation

S: F1089F

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-ΔHsFBXO28-Myc-MBP-HA

Plasmid

#228946

Purpose

Expression ΔHsFBXO28-Myc (lacking F-box domain) and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

ΔHsFBXO28 (FBXO28 Human)

Tags

myc

Expression

Bacterial

Mutation

deletion of the F-box domain

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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