We narrowed to 16,131 results for: form

1. LN-entry+NLS

   Plasmid

   #128011

   Purpose

   Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   LN-3xFlag-SV40NLS
   

   Use

   Lamdan entry vector with nls to generate tetherin…

   Tags

   LN-3xFlag-SV40NLS

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

   Plasmid

   #184334

   Purpose

   Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

   Depositor

   Peter Scheiffele

   Article

   Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

   Insert

   Nrxn3-alpha (Nrxn3 Mouse)
   

   Tags

   HA-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-BG505.DS-SOSIP.6mut-gp160

   Plasmid

   #123278

   Purpose

   Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; DS-SOSIP.6mut variant

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BG505) Env
   

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; SOSIP mutations; …

   Promoter

   CMV

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ7529 pHR (hU6-crLPT-EFS-PuroR-WPRE)

   Plasmid

   #214877

   Purpose

   Lentiviral vector encoding RfxCas13d targeting LPT guide array

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crLPT-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP-KCC2

   Plasmid

   #104075

   Purpose

   expresses rat KCC2 (b isoform) with eGFP tag at the N-terminus of KCC2

   Depositor

   Igor Medyna

   Article

   Pellegrino et al J Physiol. 2011 May 15;589(Pt 10):2475-9

   Insert

   KCC2 (b isoform) (Slc12a5 Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   EcoR1 site in rat KCC2 was removed by silent muta…

   Promoter

   CMV

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSK+ mBMPR-1B DN

   Plasmid

   #49530

   Purpose

   cloning vector for dominant negative mouse BMP receptor 1B

   Depositor

   Lee Niswander, Peter ten Dijke

   Article

   Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

   Insert

   BMPR-1B DN (Bmpr1b Mouse)
   

   Expression

   Bacterial

   Mutation

   dominant negative mutation (K231R); also L2V

   Promoter

   T3

   Available Since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pDONR223 LC3B no-stop

   Plasmid

   #123205

   Purpose

   Gateway entry clone encoding human MAP1LC3B lacking stop codon, suitable for C-terminal tagging

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Stop codon removed

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223 LC3A no-stop

   Plasmid

   #123204

   Purpose

   Gateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal tagging

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Use

   Gateway entry vector / entry clone

   Mutation

   Stop codon removed

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-PTRF

   Plasmid

   #68401

   Purpose

   Expression of fluorescently tagged PTRF in mammalian cells

   Depositor

   Rob Parton

   Article

   Hill et al Cell. 2008 Jan 11;132(1):113-24. doi: 10

   Insert

   PTRF (Cavin1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. TOPO UBC

    Plasmid

    #68456

    Purpose

    TOPO construct expressing UBC for generation of GMAP-compatible promoter

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Ubiquitin C Promoter (UBC Human)
    

    Use

    Gmap

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA

    Plasmid

    #108769

    Purpose

    Expresses N-terminus to aa396 of Flp recombinase fused to ABI CID

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

    Insert

    Flp recombinase fragment N-terminus to aa396
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    ABI,NLS

    Expression

    Mammalian

    Promoter

    pCAG

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. B1 Integrin

    Plasmid

    #186137

    Purpose

    For expression of recombinant beta1 integrin cytosolic domain in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    ITGB1 (ITGB1 Human)
    

    Tags

    MBP and untranslated His6 after stop codon

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Ncam1_1.18.1-AP-His

    Plasmid

    #71962

    Purpose

    Expresses the extracellular region of the NCAM1, isoform 1.18.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ncam1_1.18.1 (Ncam1 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Unc5c.a-Fc-His

    Plasmid

    #72180

    Purpose

    Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Unc5c.a (Unc5c Mouse)
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXS-IRES-Blast SHMT2res

    Plasmid

    #106301

    Purpose

    Expresses SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Silent mutations destroy sgRNA targeting sites

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ncam1_0.0.0-AP-His

    Plasmid

    #71960

    Purpose

    Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Ncam1_0.0.0 (Ncam1 Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET32a-Spike-403-505

    Plasmid

    #165095

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 spike protein site 403-505 (S SARS-CoV2)
    

    Expression

    Bacterial

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pMRX-IP/Venus-mATG5

    Plasmid

    #58739

    Purpose

    Expresses Venus-mATG5 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Use

    Retroviral

    Tags

    Venus

    Available Since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCMV 3xFLAG-LC3A G120A

    Plasmid

    #123090

    Purpose

    Expresses 3xFLAG-LC3A G120A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR221-CCL5.Pro

    Plasmid

    #79492

    Purpose

    MultiSite Gateway entry clones, first fragment, (attL1, attR5) CCL5/RANTES promoter

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    CCL5 Promoter (CCL5 Synthetic, Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    CCL5 Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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