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CMV-NF186(K604TAG)-HA

Plasmid

#239778

Purpose

Expresses rat NF186 with a TAG codon at position 604 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K604TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K680TAG)-HA

Plasmid

#239779

Purpose

Expresses rat NF186 with a TAG codon at position 680 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K680TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-NF186(K809TAG)-HA

Plasmid

#239780

Purpose

Expresses rat NF186 with a TAG codon at position 809 and a C-terminal HA tag in mammalian cells. Can be used for click labeling.

Depositor

Ivana Nikić-Spiegel

Article

Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

Insert

Nfasc (Nfasc Rat)

Tags

HA-tag

Expression

Mammalian

Mutation

K809TAG

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pALN130

Plasmid

#239798

Purpose

Express mEGFP tagged mouse CENPT

Depositor

Iain Cheeseman, Ally Nguyen

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

CENPT (Cenpt Mouse)

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

Moloney Murine Leukemia Virus (MMLV) LTR promoter

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pALN148

Plasmid

#239800

Purpose

Express mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domain and 4 non-synonymous substitutions

Depositor

Iain Cheeseman, Ally Nguyen

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

Mm-CENPT-Rn-Histone Fold Domain-4NS

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

4 nucleotide substitution in rat histone fold dom…

Promoter

Moloney Murine Leukemia Virus (MMLV) LTR promoter

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRps9-WT-IRES-GFP-HygR

Plasmid

#190271

Purpose

Express RPS9 WT mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

Depositor

Erik Böttger

Article

Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

Insert

ribosomal protein RPS9

Tags

IRES-eGFP

Expression

Mammalian

Promoter

CAGGS-CMV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pRps9-D95N-IRES-GFP-HygR

Plasmid

#190272

Purpose

Express RPS9 D95N-mutant mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.

Depositor

Erik Böttger

Article

Brilkova et al Cell Rep. 2022 Sep 27;40(13):111433. doi

Insert

ribosomal protein RPS9

Tags

IRES-eGFP

Expression

Mammalian

Mutation

D95N

Promoter

CAGGS-CMV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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mILK-R349A-EFGP

Plasmid

#176898

Purpose

mouse ILK with R349A point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 349 to Alanine

Promoter

CNV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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mILK-R255A-EFGP

Plasmid

#176897

Purpose

mouse ILK with R255A point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Arginine 255 to Alanine

Promoter

CNV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRD198

Plasmid

#163101

Purpose

Expression of mEGFP_H-NSdbd in Escherichia coli (and, potentially, other bacteria)

Depositor

Remus Dame

Article

Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

Insert

mEGFP_H-NSdbd

Expression

Bacterial

Promoter

araBAD promoter

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19_T790M_28bp

Plasmid

#114721

Purpose

28 bp fragment of the EGFR T790M allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Mutation

c.2369C>T (Substitution, position 2369, C➞T); …

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19_4xT790M_28bp

Plasmid

#114719

Purpose

4 x 28 bp repeats of the EGFR T790M allele used as SiMREPS analyte standard

Depositor

Nils Walter

Article

Hayward et al J Am Chem Soc. 2018 Aug 20. doi: 10.1021

Insert

EGFR (EGFR Human)

Expression

Bacterial

Mutation

c.2369C>T (Substitution, position 2369, C➞T); …

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-48-GFP

Plasmid

#108507

Purpose

expression of vsv-INCENP 1-48-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-58-GFP

Plasmid

#108508

Purpose

expression of vsv-INCENP 1-58-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Incenp 1-63-GFP

Plasmid

#108509

Purpose

expression of vsv-INCENP 1-63-EGFP

Depositor

Susanne Lens

Article

van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

Insert

CEN-Box of INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

only CEN box of INCENP

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-MYO10-BioID

Plasmid

#194858

Purpose

Express MYO10 tagged with eGFP (Nter) and BioID (Cter) in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

MYO10 (MYO10 Human)

Tags

BioID and EGFP

Expression

Mammalian

Mutation

MyoX contains a Q680R variant compared to the NCB…

Promoter

CMV

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-GABARAPL1 G116

Plasmid

#123119

Purpose

Expresses EGFP-GABARAPL1 G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

Plasmid

#168106

Purpose

PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

mCherry-KASH

Use

CRISPR and Cre/Lox

Promoter

CAG

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-DeAct-SpvB

Plasmid

#89446

Purpose

Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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CGBE1-NG (pBM1246)

Plasmid

#140256

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_NG-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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