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pYO379

Plasmid

#235733

Purpose

Protein expression of VPS34 + VPS15-ZZ + ZZ-VPS30 (all yeast) in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Tags

3xTEV-ZZ and ZZ-3xTEV

Expression

Yeast

Mutation

S2A on ScVPS34

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP-FMRP-bGH

Plasmid

#235265

Purpose

ComMAND EGFP-FMRP base gene

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_DelCTerm_1-223AA

Plasmid

#221488

Purpose

NKX2-1 C-terminal truncation mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 224-371AA C-terminal domain

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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ΔCT183-GFP

Plasmid

#33117

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the last 183 aa of the variable cytop…

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
VCDstubΔ741-752-GFP

Plasmid

#33123

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains a signal sequence fused to a very small …

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-DNAJC13(2198t+hpd)

Plasmid

#246664

Purpose

Mammalian expression of dual mutant, eGFP tagged DNAJC13

Depositor

Braden Lobingier

Article

Adoff et al Mol Biol Cell. 2025 Sep 1;36(9):ar114. d

Insert

DNAJC13 (DNAJC13 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Mutated 1332-H, 1333-P, 1334-D to Alanines; delet…

Promoter

CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235297

Purpose

ComMAND open-loop circuit regulating mRuby2, CAG-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG (synthetic cytomegalovirus early enhancer + c…

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235298

Purpose

ComMAND closed-loop circuit regulating mRuby2, CAG-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG (synthetic cytomegalovirus early enhancer + c…

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_HD_160-223AA

Plasmid

#221489

Purpose

NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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P2061

Plasmid

#186299

Purpose

Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter

Depositor

Joerg Stelling

Article

Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10

Inserts

LexA-HBD-B112
Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial and Yeast

Promoter

2xLexop-minimalCyc1 and PACT1

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Stat1Klf6

Plasmid

#188400

Purpose

Expresses mouse transcription factors Stat-1 and Klf6 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Inserts

Stat-1 (Stat1 Mouse, Escherichia coli)
Klf6 (Klf6 Mouse)

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

D-mannose

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hAP1 420-440

Plasmid

#183643

Purpose

To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 (ATP6AP1 Human)

Tags

myc

Expression

Mammalian

Mutation

truncated ATP6AP1 mutant; contains only the trans…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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CSF1R gRNA (BRDN0001146637)

Plasmid

#77038

Purpose

3rd generation lentiviral gRNA plasmid targeting human CSF1R

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CSF1R (CSF1R Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pYO391

Plasmid

#235751

Purpose

Protein expression of ScVPS38, untagged + ScVPS34, untagged + FL ScVPS15-ZZ + ZZ-FL ScVPS30

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)
VPS38 (VPS38 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Tags

3xTEV-ZZ and ZZ-3xTEV

Expression

Yeast

Mutation

S2A and T134A, I851R

Promoter

GALGAPDH

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-FXN-P2A-mRuby2-bGH

Plasmid

#235300

Purpose

ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

FXN-P2A-mRuby2 (FXN Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-[EF1a-mRuby2-bGH]-UbC-PuroR-T2A-mGL-SV40pA

Plasmid

#235325

Purpose

PiggyBac integration vector for ComMAND base gene regulating mRuby2

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH

Plasmid

#235266

Purpose

ComMAND EGFP-FMRP open-loop circuit

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-EGFP(miRE.FF4)-FMRP-TS.FF4x1-bGH

Plasmid

#235267

Purpose

ComMAND EGFP-FMRP closed-loop circuit

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

EGFP-Fmr1 (Fmr1 Mouse)

Use

Synthetic Biology

Tags

FLAG

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBoBi-hAP1-C-LAMP2 TM

Plasmid

#183645

Purpose

Lentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

ATP6AP1 and LAMP2 (ATP6AP1, LAMP2 Human)

Use

Lentiviral

Tags

HA

Mutation

ATP6AP1 transmembrane domain replaced by the tran…

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-NCOA7g3 (BB24)

Plasmid

#139459

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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