167,150 results

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pRK5-p18-FLAG

Plasmid

#42331

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 (LAMTOR1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB) (PX460)

Plasmid

#48873

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Has Service

Cloning Grade DNA

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209652

Purpose

Expression of iATPSnFR2, medium affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.HaloTag

Use

AAV

Mutation

A95K

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMIR-FLAG-MLL-AF9

Plasmid

#71444

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-AF9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-44B12mut23(Ser5ph)-SNAP

Plasmid

#174877

Purpose

PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

RNAPII Ser5ph mintbody-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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All-in-one lenti-evoCDA-NG

Plasmid

#200449

Purpose

Lentivirus vector for base editing

Depositor

Benjamin Cravatt

Article

Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pH1R-mCherry-N1

Plasmid

#84325

Purpose

Histamine-1 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H1R (HRH1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-mKeima-LGALS3

Plasmid

#175780

Purpose

Lentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic flux

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/opto-a1AR-EYFP

Plasmid

#20947

Purpose

Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Article

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-a1AR

Expression

Mammalian

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST40-MFRN2

Plasmid

#50546

Purpose

Expression of MFRN2 gene in mammalian cells

Depositor

Vamsi Mootha

Article

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

Mrfn2 (SLC25A28 Human)

Tags

V5

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
Lyso-B

Plasmid

#209873

Purpose

Dimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP B

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pERB221

Plasmid

#61502

Purpose

const. CenpB-GFP-Halo + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

CenpB-GFP-Halo
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSP64T acvr1ba*

Plasmid

#185409

Purpose

Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

acvr1ba (acvr1ba Zebrafish)

Use

In vitro synthesis of mrna

Expression

Mammalian

Mutation

changed Threonine 206 to Aspartic acid

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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Ubi:ccdb + RUBY

Plasmid

#233306

Purpose

GATEWAY compatible vector with soybean Ubiquitin (GmUbi) promoter driving in frame epitope fusion of ccdb selection toxin and RUBY marker

Depositor

Thomas Baum

Insert

Ubi:ccdb + RUBY

Use

Gateway destination

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Moesin-C-14

Plasmid

#55103

Purpose

Localization: Cytoskeleton/Membrane, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Moesin (MSN Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pTH2

Plasmid

#84452

Purpose

shuttle plasmid for sarA P1-CFP expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

CFP Cerulean

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEF274 SVAfw

Plasmid

#178192

Purpose

The SVA (SINE-VNTR-ALU) retroposon that causes human X-linked Dystonia Parkinsonism (XDP), within a BAC (pJazz) backbone.

Depositor

Elizabeth Fisher

Insert

SVA that inserts into the X-linked human TAF1 gene to cause X-linked dystonia Parkinsonism

Use

Linear bac vector

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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CLIMP-63 pcDNA

Plasmid

#80977

Purpose

mammalian expression of CLIMP-63

Depositor

Peter Steyger

Article

Karasawa et al Cell Death Dis. 2010 Nov 18;1:e102. doi:

Insert

CLIMP-63 (CKAP4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-VAMP7

Plasmid

#45913

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

vesicle-associated membrane protein 7 (Vamp7 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP-hTRF2

Plasmid

#103804

Purpose

BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-EYFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)

Expression

Mammalian

Mutation

hTRF2: deletion of amino acids 1-42 and 476 compa…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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