170,806 results

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pcDNA3.1+CMV-Ins-GLuc

Plasmid

#89929

Purpose

Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

Depositor

Melanie Cobb

Article

Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

Insert

human insulin-Gaussia-Luciferase (INS Synthetic, Human)

Expression

Mammalian

Mutation

DNA sequence for Gaussia luciferase was humanized…

Promoter

CMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWN_U6-B2M-miniGag

Plasmid

#228957

Purpose

miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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LV_EF1a_FLAG-Mkk7-JNK2-P2A-Hygro_Barcode

Plasmid

#170228

Purpose

Barcoded lentiviral vector to express Mkk7-JNK2 fusion in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

Depositor

Prashant Mali

Article

Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

Insert

Map2k7-MAPK9 fusion (MAPK9, Map2k7 Mouse, Human)

Use

Lentiviral

Tags

FLAG

Mutation

Fusion

Promoter

EF1a

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGLO-Bmpr2-1719

Plasmid

#49379

Purpose

Insert is a fragment of the Bmpr2 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.

Depositor

Martine Roussel

Article

Murphy et al Cancer Res. 2013 Oct 21.

Insert

Bmpr2 (BMPR2 Mouse, Human)

Use

Luciferase

Promoter

Human phosphoglycerate kinase (PGK) promoter

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pEA106

Plasmid

#187872

Purpose

Expresses mCherry under the virB promoter.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

mCherry

Expression

Bacterial

Promoter

Agrobacterium virB promoter

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pIVT-R2Tg-WT

Plasmid

#238105

Purpose

For IVT of wildtype R2Tg editor, codon optimized for mammalian expression.

Depositor

Feng Zhang

Article

Edmonds et al Nat Commun. 2025 Jul 2;16(1):6079. doi:

Insert

Wildtype R2Tg, mammalian codon optimized

Use

For ivt

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP21

Plasmid

#22574

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP21 (USP21 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLKO-RFP-IKZF3-sh2

Plasmid

#69043

Purpose

3rd generation lentiviral vector expressing IKZF3 shRNA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/WT M87

Plasmid

#89322

Purpose

expression of WT M87 spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted

Promoter

CMV

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_A2_F

Plasmid

#237351

Purpose

Expression plasmid for RSV A2 F

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV A2 F human codon optimized from Genbank Accesion QGW56794.1

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-PS9

Plasmid

#177944

Purpose

Transient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP (ORF1ab )

Expression

Mammalian

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pDESTpK7WG-RPS5Ap-mito

Plasmid

#163145

Purpose

Plant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter

Depositor

Shin-ichi Arimura

Article

Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

Insert

RPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG

Expression

Plant

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pFlagCMV2-UbcH8

Plasmid

#12442

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

UbcH8 (UBE2L6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
AiP1307 - pAAV-AiE2016m-minBG-SYFP2-WPRE-BGHpA

Plasmid

#214481

Purpose

AiE2016m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Blast

Plasmid

#227270

Purpose

Empty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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DL034:cFos::HaloTag-iPAK4

Plasmid

#177884

Purpose

HaloTag-iPAK4 fusion with cFos promoter, cloned into a lentivirus backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

iPAK4

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Promoter

cFos

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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Phi3-GCaMP6f

Plasmid

#106298

Purpose

CMV driven GFP based calcium (Ca2+) reporter

Depositor

Sanford Simon

Article

Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

Insert

GCaMP6f

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-NRAS(G12V)

Plasmid

#35632

Depositor

Daniel Haber

Article

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

N-Ras(G12V) (NRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pCAT201

Plasmid

#134878

Purpose

Bacterial expression

Depositor

Thomas Howard

Article

Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pML625_RTN4-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227895

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

RTN4 homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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