162,309 results

1. pHRIG-Akt1

   Plasmid

   #53583

   Purpose

   lentiviral expression of constitutively active human Akt1 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   cAkt1 (AKT1 Human)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   constitutively active (myristoylated version)

   Promoter

   CMV

   Available since

   Oct. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBHM2617

   Plasmid

   #234496

   Purpose

   NATR-CnU6-empty sgRNA

   Depositor

   Hiten Madhani

   Article

   Nalley et al bioRxiv 2025.02.14.638277

   Insert

   NAT-PCnU6-sgRNA_scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSP1D1-Strep-II

   Plasmid

   #224209

   Purpose

   Membrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.

   Depositor

   Anne Robinson

   Article

   Pettersen et al Biophys J. 2025 Jan 21;124(2):440-457. d

   Insert

   MSP1D1-Strep-II
   

   Use

   Tags

   Strep-II Tag

   Expression

   Bacterial

   Mutation

   Substitution of 7xHis tag found in pMSP1D1 for a …

   Promoter

   T7

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

   Plasmid

   #236406

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT/TO-APT2-LaG16-3W

   Plasmid

   #234425

   Purpose

   Express APT2 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT2 fused to non-binding mutant LaG16 (LYPLA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT/TO-APT1-LaG16-3W

   Plasmid

   #234424

   Purpose

   Express APT1 with a mutated non-binding anti-GFP nanobody LaG16 (F30W, T55W, G103W) fused at the C terminus separated by a flexible linker

   Depositor

   William Fuller

   Article

   Kuo et al Nat Commun. 2025 Feb 7;16(1):1445. doi:

   Insert

   APT1 fused to non-binding mutant LaG16 (LYPLA1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP12493 - pAAV-AiE0374h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2493)

   Plasmid

   #230214

   Purpose

   AiE0374h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Boaz Levi

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. T2A 3xNLS GFP

   Plasmid

   #227721

   Purpose

   Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Haosheng Sun, Oliver Hobert

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-6P-acatenin

   Plasmid

   #186458

   Purpose

   Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

   Depositor

   Tina Izard

   Article

   Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

   Insert

   CTNNA1 (CTNNA1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. DRH015_ AAV-hSyn-WT-THR

    Plasmid

    #225088

    Purpose

    Expression of wild-type thyroid receptor beta (THRB, human) with an HA tag in neurons driven by human synapsin promoter.

    Depositor

    Bernardo Sabatini

    Article

    Hochbaum et al Cell. 2024 Oct 3;187(20):5679-5697.e23.

    Insert

    WT-THRB (THRB Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDRF1 mScarletI3-T2A-mTurquoise2

    Plasmid

    #219845

    Purpose

    equimolar expression of mScarletI3 and mTurquoise2 in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mScarletI3-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pHIV-EGFP.Flag-EZH2-mt1

    Plasmid

    #220244

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 1 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R27A, R31A, F165A

    Promoter

    EF-1α

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a(+)-SUMO-A'

    Plasmid

    #209115

    Purpose

    Bacterial expression of CC-GEMS ligand SUMO-A'

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    SUMO-A'
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. GpACR1-pcDNA3.1-mScarlet

    Plasmid

    #204964

    Purpose

    Expression of GpACR1 in fusion with mScarlet in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

    Insert

    GpACR1
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV (+ enhancer)

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AiP12214 - pAAV-ProB12-SYFP2-WPRE3-BGHpA (Alias: CN2214)

    Plasmid

    #208161

    Purpose

    AAV vector for SYFP2 expression in brain astrocytes

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM33_CLIP_CAP_Gly

    Plasmid

    #178470

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    CLIP_CAP_Gly (CLIP1 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HA-DEPTOR S286/287/291A

    Plasmid

    #159160

    Purpose

    Expresses DEPTOR S286/287/291A mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed serine 286,287 and 291 to alanine

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-HA-DEPTOR S286/287/291D

    Plasmid

    #159161

    Purpose

    Expresses DEPTOR S286/287/291D mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

    Insert

    DEPTOR (DEPTOR Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed serine 286,287 and 291 to aspartic acid

    Promoter

    CMV

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p206_LTJ_sgRNACD45.2_R3

    Plasmid

    #82674

    Purpose

    sgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.

    Depositor

    Lukas Jeker

    Article

    Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

    Insert

    sgRNA targeting CD45.2 region 3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMV_RVFV_GP

    Plasmid

    #207304

    Purpose

    Mammalian expression of Rift valley fever virus GP protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    RVFV_GP (RVFV_sM_gp1 Rift valley fever virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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