PurposeBidirectional CAG promoter (compatible with the MXS Chaining Kit)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||78175||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonemodified MXS_chaining vector Addgene 62394
- Backbone size w/o insert (bp) 1930
- Total vector size (bp) 6230
Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameBidirectional CAG Promoter
Insert Size (bp)4280
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer TTACCGCCTTTGAGTGAG
- 3′ sequencing primer TTGTCTCATGAGCGGATAC (Common Sequencing Primers)
Due to the repeated CAG promoter sequences, a SpeI + EcoRI digest is recommended to confirm the integrity of the chicken-actin/beta globin promoter fragments. Please see link to a diagnostic digest image in the Resource Information section above.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MXS_BidirectionalCAG was a gift from Pierre Neveu (Addgene plasmid # 78175 ; http://n2t.net/addgene:78175 ; RRID:Addgene_78175)
For your References section:Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells. Sladitschek HL, Neveu PA. PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016. PONE-D-16-09196 [pii] PubMed 27152616