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MXS_BidirectionalCAG
(Plasmid #78175)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 78175 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    modified MXS_chaining vector Addgene 62394
  • Backbone size w/o insert (bp) 1930
  • Total vector size (bp) 6230
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bidirectional CAG Promoter
  • Species
    Synthetic
  • Insert Size (bp)
    4280

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer TTACCGCCTTTGAGTGAG
  • 3′ sequencing primer TTGTCTCATGAGCGGATAC
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Due to the repeated CAG promoter sequences, a SpeI + EcoRI digest is recommended to confirm the integrity of the chicken-actin/beta globin promoter fragments. Please see link to a diagnostic digest image in the Resource Information section above.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MXS_BidirectionalCAG was a gift from Pierre Neveu (Addgene plasmid # 78175 ; http://n2t.net/addgene:78175 ; RRID:Addgene_78175)
  • For your References section:

    Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells. Sladitschek HL, Neveu PA. PLoS One. 2016 May 6;11(5):e0155177. doi: 10.1371/journal.pone.0155177. eCollection 2016. PONE-D-16-09196 [pii] PubMed 27152616