169,328 results
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Plasmid#158398PurposeGolden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterWithout promoterAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only
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YopH (residues 164-468)
Plasmid#79749PurposeThis plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.DepositorInsertYopH (residues 164-468)
ExpressionBacterialPromoterT7Available SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
10XSTAT92E–luciferase
Plasmid#37393DepositorInsertSOCS36E enhancer fragment (Socs36E Fly)
UseLuciferase; Jak/stat reporter constructExpressionInsectPromoterHsp70Available SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-NE2h
Plasmid#208687PurposeExpresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neuronsDepositorHas ServiceAAV5InsertGPCR activation based norepinephrine (NE) sensor GRAB_NE2h
UseAAVPromoterhSynAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TDP-REGv1(mCherry-FLAG) in pTwist-CMV
Plasmid#216165PurposeExpression of mCherry specifically in cells with TDP-43 knockdown. Uses AARS1-derived cryptic exon to control mCherry expression. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor CommentsDepositorInsertmCherry with C-terminal FLAG tag
ExpressionMammalianAvailable SinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-S2215Y
Plasmid#69013Purposeactivating MTOR mutationDepositorAvailable SinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
GFP-ERK2 K52R
Plasmid#118298PurposeExpression of Rat ERK2DepositorAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymScarletI
Plasmid#118452PurposeOverexpression of yeast codon-optimized mScarletIDepositorInsertymScarletI
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Human RNF144A (16-228)
Plasmid#110752PurposeBacterial Expression of RNF144A RBR domainDepositorAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE14
Plasmid#90349PurposeVDR - Vitamin D receptor gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterVDRAvailable SinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TID-HA3-HIS3MX6
Plasmid#227981PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, HIS3MX6 markerDepositorInsertTurboID
TagsHA tagExpressionYeastAvailable SinceNov. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR
Plasmid#108104PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation)PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CKIIa-stGtACR2-FusionRed
Plasmid#105669PurposeExpresses a soma-targeted GtACR2 in mammalian neurons under the CaMKIIa promoterDepositorHas ServiceAAV Retrograde and AAV1InsertstGtACR2
UseAAVTagsFusionRed and Kv2.1ExpressionMammalianPromoterCaMKIIaAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-GGamma12-GFP2
Plasmid#166781PurposeEncodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGGamma12-GFP2 (GNG12 Synthetic, Human)
ExpressionMammalianMutationContains an N-terminal GFP2 and GSAG linkerPromoterCMVAvailable SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCX-EGFP c-Abl
Plasmid#15007DepositorAvailable SinceAug. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
LeGO-G/BSD
Plasmid#27354DepositorInsertU6 promoter, SFFV promoter, eGFP/BSD-Fusion
UseLentiviralExpressionMammalianAvailable SinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_K95M
Plasmid#108106PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable SinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0765
Plasmid#133501PurposeExpression of mCherry targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM427
Plasmid#210788PurposeL5 integrating vector for delivery of defective Hyg gene (ZeoR) for oligonucleotide-mediated SNP transferDepositorInsertDefective hygromycin resistant gene
ExpressionBacterialMutationChanged hygromycin resistant gene W190 codon (TGG…Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUGI-NLS
Plasmid#101091PurposeExpresses UGI in mammalian cellsDepositorInsertUGI
TagsNLSExpressionMammalianPromoterCMVAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only