164,718 results

1. pLV-TetO-hNGN2-Neo

   Plasmid

   #99378

   Purpose

   Expresses human NEUROGENIN2 (hNGN2) and neomycin resistance gene under control of TetON promoter. 3rd generation lentiviral backbone.

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   hNGN2-P2A-Neo (NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-TGFBR2

   Plasmid

   #23623

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TGFBR2 (TGFBR2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1+ eiF4A1 myc HIS

   Plasmid

   #71657

   Purpose

   mammalian expression of eiF4A1

   Depositor

   Gideon Dreyfuss

   Insert

   eiF4A1 (EIF4A1 Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   aa403 from Ala to Arg

   Promoter

   CMV

   Available Since

   Jan. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458M-53BP1-DN1S

   Plasmid

   #131045

   Purpose

   Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

   Depositor

   Punam Malik

   Article

   Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

   Insert

   SpCas9-53BP1-DN1S (TP53BP1 Human)
   

   Use

   CRISPR

   Tags

   GFP and SpCas9

   Expression

   Mammalian

   Mutation

   Only expressing residues 1231-1644 of complete 53…

   Promoter

   CBH

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. JC Mad-1

   Plasmid

   #25626

   Depositor

   Peter Howley

   Article

   Howley et al J Virol. 1980 Dec . 36(3):878-82.

   Insert

   JC Virus Mad-1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pLV-CMV-dnMCAK

   Plasmid

   #205993

   Purpose

   Over-expression of GFP-dnMCAK

   Depositor

   Floris Foijer

   Article

   Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

   Insert

   dnMCAK
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Sec23A

   Plasmid

   #66609

   Purpose

   GFP fusion of Sec23A

   Depositor

   David Stephens

   Article

   Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

   Insert

   Sec23A (SEC23A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV

   Plasmid

   #24828

   Depositor

   Lin He

   Article

   Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG

   Plasmid

   #148828

   Purpose

   Mammalian Expression of HsNot9-shRNA2res

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

   Insert

   HsNot9-shRNA2res (CNOT9 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX_DSB_Spectrum-V2

    Plasmid

    #192475

    Purpose

    Lentiviral DSB Spectrum V2 Reporter System

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    BFP1.2, incompleteGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMito-TrxRFP1

    Plasmid

    #98997

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and mitochondrial localization sequence
    

    Use

    Tags

    His-tag and Mitochondria localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PEmax-SpG

    Plasmid

    #211549

    Purpose

    Prime editing in mammalian cells

    Depositor

    Yongsub Kim

    Article

    Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

    Insert

    PEmax-SpG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. FL-fibulin-3-V5-lentiviral-GFP

    Plasmid

    #29703

    Depositor

    Mariano Viapiano

    Article

    Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

    Insert

    EFEMP1 (EFEMP1 Human)
    

    Use

    Lentiviral

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pDUAL CD81 (GFP)

    Plasmid

    #86980

    Purpose

    Lentiviral expression construct encoding CD81 and GFP from separate promoters

    Depositor

    Joe Grove

    Insert

    CD81 (CD81 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV/hPGK

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. LRCherry2.1

    Plasmid

    #108099

    Purpose

    Lentiviral expression plasmid of sgRNA with mCherry

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for sgRNA expression and EFS promoter…

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18-mini-Tn7T-Gm-lacZ

    Plasmid

    #63120

    Purpose

    mini-Tn7 lacZ transcriptional fusion vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. luxB:cp157Venus+luxA/pRSETB Duet

    Plasmid

    #197583

    Purpose

    BRET lux luciferase with cp157Venus for bacterial expression

    Depositor

    Takeharu Nagai

    Article

    Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

    Insert

    LuxA LuxBcp157Venus
    

    Use

    E.coli expression

    Tags

    His X 6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBZCas13b

    Plasmid

    #89898

    Purpose

    Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

    Depositor

    Feng Zhang

    Article

    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

    Insert

    Cas13b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-GRAB-rDA2h

    Plasmid

    #208701

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h in neurons

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_rDA2h
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ube1/PET21d

    Plasmid

    #34965

    Depositor

    Cynthia Wolberger

    Article

    Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

    Insert

    Ube1 (UBA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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