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pNCS-mRuby3

Plasmid

#74234

Purpose

Constitutive expression of mRuby3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mRuby3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-1a

Plasmid

#26801

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1a (SREBF1 Human)

Tags

2xFLAG

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_CD63-SBP-EGFP

Plasmid

#247324

Purpose

Synchronize the trafficking of CD63 from the ER.

Depositor

Franck Perez, Clotilde Thery

Article

Mathieu et al Nat Commun. 2021 Jul 19;12(1):4389. doi:

Insert

CD63 (CD63 Human)

Tags

SBP-EGFP (inserted in the small luminal loop of C…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLEX SC4MOL

Plasmid

#27983

Depositor

Igor Astsaturov

Insert

SC4MOL (MSMO1 Human)

Use

Lentiviral

Tags

HA and MYC

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pICE-EGFP-FLAG-PSMD2

Plasmid

#161917

Purpose

Expresses human PSMD2 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

26S proteasome non-ATPase regulatory subunit 2 (PSMD2 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCerulean-N1 Cyclin B1 (1147)

Plasmid

#61852

Purpose

mammalian expression of Cyclin B1 fused to Cerulean

Depositor

Jonathon Pines

Insert

Cyclin B1 (CCNB1 Human)

Tags

Cerulean

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
spNW25

Plasmid

#173484

Purpose

Expresses spNW25 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW25

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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p13xQUAS-ZsGreen-P2A, α-cry:mCherry

Plasmid

#180480

Purpose

Inducible gene expression vector p13xQUAS-ZsGreen-P2A, α-cry:mCherry

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

13x QUAS-ZsGreen-P2A

Use

Synthetic Biology

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pPlin5-mScarlet3-H (aka mYongHong)

Plasmid

#239408

Purpose

Expresses Plin5-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

Plin5 (PLIN5 Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_RPS6KB1_WT

Plasmid

#82161

Purpose

Gateway Donor vector containing RPS6KB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RPS6KB1 (RPS6KB1 Human)

Use

Gateway Cloning

Mutation

P262H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV_ABEmax

Plasmid

#112095

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUK21

Plasmid

#49788

Purpose

E. coli cloning vector (KanR, high copy, blue/white selection, M13 IR)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Promoter

lacZ

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pET-TP1107(Q15TAG)

Plasmid

#247462

Purpose

Bacterial expression of anti-IgG VHH clone TP1107 with amber stop codon at Q15 for unnatural amino acid incorporation

Depositor

Angus Johnston

Article

Chen et al Nat Nanotechnol. 2025 Sep;20(9):1273-128

Insert

TP1107 Q15TAG

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Mutation

Original Q15 codon (CAA) was modified to amber st…

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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PGK-DTAG-NTERM-GFP

Plasmid

#185775

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, dTAG

Mutation

none

Promoter

PGK

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pTK_Sox9_enh_366_Citrine

Plasmid

#130593

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Sox9_enh_366

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF2029

Plasmid

#143566

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SMARCA5 (SMARCA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLGB36

Plasmid

#135621

Purpose

Suicide vector for allelic replacement in Bacteroides species including B. fragilis strain 638R, erythromycin selection and aTC-inducible ss-Bfe3 counterselection

Depositor

Laurie Comstock

Article

Ito et al mBio. 2020 Feb 4;11(1). pii: mBio.03238-

Insert

bfe3

Use

Bacteroides suicide vector with inducible counter…

Tags

signal sequence for periplasmic targeting

Promoter

Bacteroides promoter regualted by TetR transcript…

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.iGluSnFR3.v857.PDGFR

Plasmid

#178333

Purpose

Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

Depositor

Kaspar Podgorski

Article

Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

Insert

pAAV CAG iGluSnFR3 v857.PDGFR

Use

AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

Tags

IgK-chain, Myc epi tag, and PDGFR TM Domain

Expression

Mammalian

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-EEA1

Plasmid

#42635

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

EEA1 (EEA1 Human)

Tags

TagRFP-T

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-FOXA1

Plasmid

#153109

Purpose

Expression of Flag tagged human FOXA1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXA1 (FOXA1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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