166,289 results

1. GFP-Rab27A

   Plasmid

   #89237

   Purpose

   Expression of GFP-tagged human Rab27A in mammalian cells

   Depositor

   William Gahl

   Article

   Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

   Insert

   human Rab27A (RAB7A Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCROPseq-VEX-optimized-scaffold

   Plasmid

   #203321

   Purpose

   Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJet1.2 kanR cassette

   Plasmid

   #117122

   Purpose

   Kanamycin resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Kanamycin resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC.HA Vav2

   Plasmid

   #14554

   Depositor

   Joan Brugge

   Article

   Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

   Insert

   Vav2 (VAV2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSFDuet-1_DNAseI

   Plasmid

   #182408

   Purpose

   Bacterial expression of DNaseI

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   DNase I (DNASE1 Bovine)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGMC00025 (aka pCW-dCas9-Blast)

   Plasmid

   #195316

   Purpose

   Dox inducible lentiviral dead Cas9

   Depositor

   Raj Chari

   Insert

   dead Cas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Bacterial and Mammalian

   Mutation

   D10A; H840A

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+cPLA2-mKate2

   Plasmid

   #162068

   Purpose

   Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

   Depositor

   Philipp Niethammer

   Article

   Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

   Insert

   cytosolic phospholipase a2 (pla2g4aa Zebrafish)
   

   Use

   Avian, xenopus, zebrafish

   Tags

   mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDisplay-pHoran4

   Plasmid

   #61557

   Purpose

   Orange fluorescent protein based pH indicator in pDisplay vector

   Depositor

   Robert Campbell

   Article

   Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

   Insert

   pHoran4
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

   Plasmid

   #159918

   Purpose

   Mutagenesis of Drd2

   Depositor

   Larry Zweifel

   Insert

   Drd2 (Drd2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-puro-N-Flag-h-SNRNP40

    Plasmid

    #134254

    Purpose

    Lentivector encoding Flag-tagged human SNRNP40

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    SNRP40 (SNRNP40 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3puro-mGFP

    Plasmid

    #225446

    Purpose

    Express mEGFP-fusion protein of interest

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Type

    Empty backbone

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-HDAC4

    Plasmid

    #45636

    Depositor

    Tso-Pang Yao

    Article

    Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

    Insert

    HDAC4 (HDAC4 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP shRNA tet pLKO puro

    Plasmid

    #163017

    Purpose

    Control tet-inducible shRNA targeting eGFP

    Depositor

    Mark Rubin

    Article

    Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Flag-FOXG1

    Plasmid

    #153124

    Purpose

    Expression of Flag tagged human FOXG1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXG1 (FOXG1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti PGK V5-LUC Puro (w543-1)

    Plasmid

    #19360

    Purpose

    3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. phND2-N174

    Plasmid

    #31822

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    NeuroD2 (NEUROD2 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5_mEGFP-NPM1

    Plasmid

    #237667

    Purpose

    For overexpression of mEGFP-NPM1

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-Cyto-CaNAR2

    Plasmid

    #64729

    Purpose

    Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) phosphatase activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    CaNAR2-NES
    

    Tags

    Cerulean3, Nuclear export signal (NES), and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Mammalian_SpCas9_Target-AID3S

    Plasmid

    #188649

    Purpose

    Expresses SpCas9_Target-AID3S in mammalian cells

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Insert

    nCas9-AID3S-UGI
    

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SpCas9

    Promoter

    pCMV

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF0591

    Plasmid

    #142654

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXO4 (FOXO4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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