164,718 results

1. pMRXIP VPS16-GFP

   Plasmid

   #67023

   Purpose

   Human VPS16 ORF was inserted into pMRXIP GFP-N vector

   Depositor

   Noboru Mizushima

   Article

   Jiang et al Mol Biol Cell. 2014 Apr;25(8):1327-37. d

   Insert

   Homo sapiens vacuolar protein sorting 16 homolog (S. cerevisiae) (VPS16) (VPS16 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pSB700 lenti gRNA Blasto

   Plasmid

   #167904

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. SRPK1 (SRPK1A-c100)

   Plasmid

   #98246

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   SRPK1A (SRPK1 Human)
   

   Use

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRL056 pDESTattB_exorh:EGFP

   Plasmid

   #213510

   Purpose

   attB-containing pDEST plasmid, for use in Multisite Gateway reaction

   Depositor

   Christian Mosimann

   Article

   Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-DIO-mCherry-WPRE-pA

   Plasmid

   #37083

   Purpose

   Cre-dependent expression of mCherry

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   mCherry
   

   Use

   AAV and Cre/Lox; Cre-on

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. PlepA

   Plasmid

   #55175

   Purpose

   Strong constitutive promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   lepA promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGal4BD-CRY2

   Plasmid

   #28243

   Purpose

   Expresses Gal4 DNA Binding Domain fused to CRY2 for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   Gal4BD

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

   Plasmid

   #128164

   Purpose

   UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).

   Depositor

   Marcus Noyes

   Article

   Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

   Insert

   10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
   

   Use

   Synthetic Biology

   Tags

   FLAG and zif268

   Expression

   Bacterial

   Mutation

   Promoter

   UV5

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLdCN

   Plasmid

   #84290

   Purpose

   Express gRNA and Cas9 in Leishmania with Neomycin resistance

   Depositor

   Greg Matlashewski

   Article

   Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

   Insert

   gRNA and Cas9
   

   Use

   CRISPR; Leishmania

   Tags

   Expression

   Mutation

   Promoter

   L. donovani ribosome RNA promoter

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET11a+CYP119

    Plasmid

    #66131

    Purpose

    Expresses the wild type of CYP119

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid

    #128163

    Purpose

    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

    Depositor

    Marcus Noyes

    Article

    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

    Insert

    ERBIN PDZ domain (ERBIN Synthetic)
    

    Use

    Synthetic Biology

    Tags

    FLAG and Omega subunit of RNA polymerase

    Expression

    Bacterial

    Mutation

    Promoter

    UV5

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-TGNP-N-10

    Plasmid

    #55145

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TGNP (TGOLN2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Talin head-SpyCatcher003

    Plasmid

    #133566

    Purpose

    Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    EGFP-Talin head-SpyCatcher003
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. FUW-RLuc-T2A-PuroR

    Plasmid

    #102320

    Purpose

    expression of r-luciferase

    Depositor

    Rudolf Jaenisch

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    Renilla luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ubc

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. OmpA3-PNGaseF-TEV-His6

    Plasmid

    #114274

    Purpose

    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

    Depositor

    Martin Caffrey

    Insert

    Peptide: N-glycosidase F
    

    Use

    Tags

    OmpA3 leader sequence (for periplasmic localisati…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-SPNS1_STOP

    Plasmid

    #161439

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SPNS1 (SPNS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pFETCh_RAD21

    Plasmid

    #64056

    Purpose

    Homology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RAD21 Homology Arms (RAD21 Human)
    

    Use

    Tags

    3X Flag P2A Neo

    Expression

    Mutation

    Promoter

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-CCR5

    Plasmid

    #98943

    Purpose

    Mammalian expression plasmid for untagged human CCR5

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-ASAP3ER

    Plasmid

    #195356

    Purpose

    ASAP3ER under the control of the pCAG promoter

    Depositor

    Brenda Bloodgood

    Article

    Campbell et al Cell Rep. 2023 Jan 31;42(1):111943. doi:

    Insert

    ASAP3ER
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-YFP-KRAS4B

    Plasmid

    #112718

    Purpose

    Expresses YFP-KRAS4B fusion protein used for FRET studies in eukaryotic cells.

    Depositor

    Kenneth Westover

    Article

    Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

    Insert

    YFP-KRAS4B (KRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge