159,557 results

1. Lenti_gRNA-Puro

   Plasmid

   #84752

   Purpose

   Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. GLP1R-Tango

   Plasmid

   #66295

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GLP1R (GLP1R Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVHA SUZ12

   Plasmid

   #24232

   Purpose

   Mammalian expression of human SUZ12 with HA tag

   Depositor

   Kristian Helin

   Article

   Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

   Insert

   SUZ12 (SUZ12 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-HA-USP3

   Plasmid

   #22582

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP3 (USP3 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmScarlet-i_C1

   Plasmid

   #85044

   Purpose

   Mammalian expression of mScarlet-i

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   mScarlet-i
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bidirectional MYC 5'UTR Dual Fluorescence Reporter

   Plasmid

   #229498

   Purpose

   This is a lentiviral reporter for translation initiation under the control of the Myc 5'UTR, which produces destabilized GFP (dsGFP). There is a control destabilized mCherry (dsmCherry) as a control.

   Depositor

   Davide Ruggero

   Article

   Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

   Insert

   MYC 5'Untranslated region + destabilized GFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   Feb. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Klb-EGFP

   Plasmid

   #45531

   Depositor

   Zipora Yablonka-Reuveni

   Article

   Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

   Insert

   beta Klotho (Klb Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX401-INK4A

   Plasmid

   #121919

   Purpose

   Doxycycline-inducible expression of p16-INK4A product of CDKN2A

   Depositor

   William Hahn

   Article

   Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

   Insert

   p16-INK4A (CDKN2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE Tet ON

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pME-Cas9-T2A-GFP

   Plasmid

   #63155

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDB4695

    Plasmid

    #171127

    Purpose

    A plasmid containing the codon-optimized OsTIR1-F74A, which is under the control of adh1 promoter. It served as one of the F-box protein expression plasmids in AID system.

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2022 Jan 4;12(1):jkab393.

    Insert

    OsTIR1-F74A
    

    Use

    Other

    Tags

    Expression

    Mutation

    non applicable

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

    Plasmid

    #37120

    Purpose

    DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    TdTomato-EGFP
    

    Use

    AAV and Cre/Lox; Cre-switch

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH2B-miRFP703

    Plasmid

    #80001

    Purpose

    Histone 2B labeling with near-infrared fluorescent protein miRFP703

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    histone cluster 1 H2B family member j (H2BC11 Human)
    

    Use

    Tags

    miRFP703

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXJ42-p200 CUX1

    Plasmid

    #100813

    Purpose

    Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

    Depositor

    Alain Nepveu

    Article

    Wilson et al J Biol Chem. 2009 Jan 2;284(1):36-45. do

    Insert

    p200 CUX1 (CUX1 Human)
    

    Use

    Note that this plasmid does not have an sv40 ori

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAMD1-4

    Plasmid

    #61565

    Purpose

    Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. p6XHis_NLS-SaCas9

    Plasmid

    #101086

    Purpose

    Express SaCas9 in bacteria with a 6xHis tag for purification

    Depositor

    Rick Tarleton

    Article

    Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

    Insert

    CRISPR-associated protein Cas9/Csn1
    

    Use

    CRISPR

    Tags

    TrxA-6xHis-STag

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 HA C9ORF72 op

    Plasmid

    #74156

    Purpose

    Express HA tagged optimized C9ORF72 in mammalian cells

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    C9ORF72 (C9orf72 Synthetic, Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    optimized sequence

    Promoter

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV

    Plasmid

    #24828

    Depositor

    Lin He

    Article

    Olive et al Genes Dev. 2009 Dec 15. 23(24):2839-49.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. USP25_pCSdest

    Plasmid

    #53817

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    USP25 (USP25 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

19. CD9-mEGFP

    Plasmid

    #182864

    Purpose

    Localization: Tetraspanin/Membrane

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    human CD9 (CD9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lyso-B

    Plasmid

    #209873

    Purpose

    Dimerization dependent fluorescent protein B anchored to cytosolic face of the lysosomal membrane with human LAMP1

    Depositor

    Sarah Cohen

    Article

    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

    Insert

    ddGFP B
    

    Use

    Tags

    LAMP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge