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pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-ERBB2

Plasmid

#23888

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ERBB2 (ERBB2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
CAG-FLEX-MCS-WPRE-SV40 - GCH2

Plasmid

#230815

Purpose

AAV cloning vector for CRE-dependent expression under the CAG promoter.

Depositor

Kimberly Ritola

Type

Empty backbone

Use

AAV and Cre/Lox; Cloning vector

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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AAT-PB-CG2APtk

Plasmid

#86003

Purpose

homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

eGFP_T2A_puro_dTK

Use

Piggybac exciseable

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pSCD-TEVP

Plasmid

#226225

Purpose

Express TEVP

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

TEVP (TEVgp1 Tobacco etch virus)

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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UBC plasmid

Plasmid

#169746

Purpose

Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRosa26PAm1 (DM#272)

Plasmid

#15036

Depositor

Douglas Melton

Article

Murtaugh et al Proc Natl Acad Sci U S A. 2003 Dec 9. 10

Insert

pRosa26 modified targeting vector

Use

For making transgenic constructs

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp3-myc

Plasmid

#11813

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 3 (CASP3 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Lifeact-EGFP

Plasmid

#58470

Purpose

Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

Depositor

Dyche Mullins

Article

Belin et al Bioarchitecture. 2015 Aug 28:1-14.

Insert

Lifeact

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mNeptune2.5

Plasmid

#197197

Purpose

Expresses the protein of mNeptune2.5 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mNeptune2.5

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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prhaBAD-anti-rabbit-Nb-Hia5

Plasmid

#239627

Purpose

Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

Depositor

Aaron Straight

Article

Sidhwani et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

Hia5

Tags

6HIS, MBP, TEV, and anti-Rabbit Nanobody

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUAST-2x attB

Plasmid

#206362

Purpose

includes two standard UAS cassettes and a third cassette without a promoter

Depositor

Erdem Bangi

Article

Quintero et al Cell Rep. 2023 Nov 28;42(11):113370. doi

Type

Empty backbone

Expression

Insect

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-EGFP

Plasmid

#164940

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pT2SC

Plasmid

#59382

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-cat

Use

Template

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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GAL4-DrBphP

Plasmid

#158986

Purpose

Expresses DrBphP fused with an N-terminal GAL4 DNA binding domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

GAL4-DrBphP

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-IRES-Neo

Plasmid

#28019

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Type

Empty backbone

Expression

Mammalian

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pRS143

Plasmid

#122377

Purpose

CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pB-Neo-rTOM20-TagBFP

Plasmid

#210332

Purpose

Overexpression of rTOM20

Depositor

Li Yu

Article

Jiao et al Cell. 2021 May 27;184(11):2896-2910.e13.

Insert

rTOM20 (Tomm20 Rat)

Tags

TagBFP

Expression

Mammalian

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189923

Purpose

AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e V106W

Use

AAV

Mutation

nSaCas9 KKH, TadA ABE8e V106W

Promoter

EFS

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pUA66 theta GFP

Plasmid

#105607

Purpose

Expresses GFP with no promoter

Depositor

Pamela Silver

Article

Stirling et al Mol Cell. 2017 Nov 16;68(4):686-697.e3.

Insert

GFP

Expression

Bacterial

Promoter

No Promoter

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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