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HaloTag-A2A-adenosine receptor
(Plasmid #66995)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66995 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    Derived from pHT2
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3988
  • Total vector size (bp) 6286
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HaloTag-A2A-adenosine receptor
  • Alt name
    ADORA2A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2298
  • Mutation
    The signal sequence from human growth hormone was added to the N-terminus of HaloTag, which was then added to the amino terminus of the A2A-adenosine receptor.
  • Entrez Gene
    ADORA2A (a.k.a. A2aR, ADORA2, RDC8)
  • Promoter CMV
  • Tag / Fusion Protein
    • Human Growth hormone Signal Sequence- HaloTag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer EBV Reverse
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A2A-adenosine receptor cDNA was purchased from Guthrie cDNA Resource Center, Sayre PA (now Missouri S&T cDNA Resource Center, Rolla, MO). HaloTag pHT2 was purchased from Promega.
  • Terms and Licenses

Depositor Comments

A2A-adenosine receptor insert (without the signal sequence) can be retrieved using Ngo MIV.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HaloTag-A2A-adenosine receptor was a gift from Catherine Berlot (Addgene plasmid # 66995 ; http://n2t.net/addgene:66995 ; RRID:Addgene_66995)