162,325 results

1. pJRK261

   Plasmid

   #173754

   Purpose

   CeLINC plasmid

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   (empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHC79

   Plasmid

   #167551

   Purpose

   Backbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213

   Depositor

   Renato Morona

   Article

   Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

   Type

   Empty backbone

   Use

   Cosmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK122 pDESTattB_exorh:mCherry

   Plasmid

   #213511

   Purpose

   attB-containing pDEST plasmid, for use in Multisite Gateway reaction

   Depositor

   Christian Mosimann

   Article

   Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-IDH2-WT

   Plasmid

   #87926

   Purpose

   Expresses IDH2 (wild-type) in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

   Insert

   isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-VSP4-E228Q

   Plasmid

   #80351

   Purpose

   Expression of GFP-tagged, dominant negative VPS4 mutant

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   VPS4-E228Q (VPS4A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSW036

   Plasmid

   #140034

   Purpose

   KO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoter

   Depositor

   Peter Nixon

   Article

   Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

   Insert

   Yellow fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   cpt

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLBS-mScarlet

   Plasmid

   #129337

   Purpose

   cytosol marker, lentivirus

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   mScarlet (Addgene 85042)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-cT3G-cERP2-NR5A1

   Plasmid

   #192915

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   NR5A1 (NR5A1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-FLAG-BMAL1-S513A/S515A/S516A

   Plasmid

   #186835

   Purpose

   Expresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Serine 513/515/516 to Alanine

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBABEpuro-ERBB2

    Plasmid

    #40978

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTH2

    Plasmid

    #84452

    Purpose

    shuttle plasmid for sarA P1-CFP expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    CFP Cerulean
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. BirA pLEMO

    Plasmid

    #119818

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Rhamnose promoter

    Available since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_DmKHC[1-421]-1xGFP-6xHis

    Plasmid

    #196972

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    6xHis and GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. Green cGull

    Plasmid

    #86867

    Purpose

    Green fluorescent probe for cGMP

    Depositor

    Tetsuya Kitaguchi

    Article

    Matsuda et al ACS Sens. 2017 Jan 27;2(1):46-51. doi: 1

    Insert

    Green cGull
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPomyces-Sth1Cas9

    Plasmid

    #129552

    Purpose

    Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    Sth1Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)-HA-Flag-natT1R2

    Plasmid

    #113944

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-839

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMRX-INU-FLAG-FAM134B

    Plasmid

    #128260

    Purpose

    Expresses FAM134B in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    FAM134B (RETREG1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-mPCSK4

    Plasmid

    #122675

    Purpose

    Expresses mouse PCSK4 in mammalian cells

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus proprotein convertase subtilisin/kexin type 4 (Pcsk4) (Pcsk4 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ADRA1B-Tango

    Plasmid

    #66214

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADRA1B (ADRA1B Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge