159,557 results

1. pZac2.1-GfaABC1D-TurboID(C )-HA-GPI

   Plasmid

   #163697

   Purpose

   Expresses c-terminal half of TurboID on surface of astrocytes

   Depositor

   Scott Soderling

   Article

   Takano et al Nature. 2020 Dec;588(7837):296-302. doi:

   Insert

   GPI anchored TurboID
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. iRFP-PH-PLCdelta1

   Plasmid

   #66841

   Purpose

   expression of near-infrared PI(4,5)P2 biosensor

   Depositor

   Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   PH-PLCdelta1
   

   Use

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-myc-BirA*-KRASG12V

   Plasmid

   #122208

   Purpose

   Expresses myc-BirA* fused to KRASG12V in mammalian cells

   Depositor

   Christopher Counter

   Article

   Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

   Insert

   KRASG12V (KRAS Human)
   

   Use

   Tags

   myc-BirA*

   Expression

   Mammalian

   Mutation

   changed G12 to V

   Promoter

   CMV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. SP6-VEE-GFP

   Plasmid

   #58976

   Purpose

   expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

   Depositor

   Steven Dowdy

   Article

   Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

   Insert

   EGFP
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   26S subgenomic promoter

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. VEGFR2-mCh

   Plasmid

   #108854

   Purpose

   Encodes for human VEGFR2 fluorescently labeled with mCherry on the C-Terminus via a 3 amino acid (GGS) flexible linker

   Depositor

   Kalina Hristova

   Article

   Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

   Insert

   KDR (KDR Human)
   

   Use

   Tags

   Labeled with mCherry on the C-Terminus via a 3 am…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2FLAG

   Plasmid

   #16331

   Depositor

   Peter Klein

   Insert

   Flag tag
   

   Use

   Vertebrate expression

   Tags

   Flag

   Expression

   Mutation

   Flag tag inserted into pCS2+ polylinker

   Promoter

   Available since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET32a-HD46Q

   Plasmid

   #11515

   Depositor

   Pamela Bjorkman

   Article

   Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

   Insert

   huntingtin, exon 1, 46Q glutamines (HTT Human)
   

   Use

   Tags

   His, His (out of frame), and Trx

   Expression

   Bacterial

   Mutation

   huntingtin exon 1 with 46 glutamines, vector m…

   Promoter

   Available since

   April 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDMyc-TNPO1

   Plasmid

   #86081

   Purpose

   A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Transportin-1 (TNPO1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLQ7558 pHR (hU6-crB2M-EFS-PuroR-WPRE)

   Plasmid

   #214881

   Purpose

   Lentiviral vector encoding RfxCas13d targeting B2M guide

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   hU6-crB2M-EFS-PuroR-WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-PPARgamma2

    Plasmid

    #78768

    Purpose

    To overexpress PPARgamma2 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Kim et al Cell Death Differ. 2014 Apr;21(4):594-60

    Insert

    PPARG (Pparg Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

11. CALM-pmCherryN1

    Plasmid

    #27691

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    CALM (Picalm Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHIV-BRAFV600E-mOrange2

    Plasmid

    #110732

    Purpose

    Mammalian Expression of BRAFV600E IRES H2B-mOrange2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    BRAFV600E IRES H2B-mOrange2 (BRAF Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    BRAF V600E

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. V48 ELP

    Plasmid

    #68395

    Purpose

    Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

    Depositor

    Andrew MacKay

    Article

    Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

    Insert

    V48 ELP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-Nluc

    Plasmid

    #180501

    Purpose

    Mammalian expression of Nluc

    Depositor

    Masaharu Somiya

    Article

    Somiya et al bioRxiv 2022.01.25.477620

    Insert

    Nluc
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENN.AAV.cTNT.PI.eGFP.WPRE.rBG

    Plasmid

    #105543

    Purpose

    AAV expression of EGFP from cTNT promoter

    Depositor

    James M. Wilson

    Has Service

    AAV9

    Insert

    cTNT-PI-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cTNT

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV-hGFAP-GFP

    Plasmid

    #183906

    Purpose

    Expression of GFP under the human GFAP ABC1D promoter

    Depositor

    Chun-Li Zhang

    Article

    Niu et al Nat Cell Biol. 2013 Oct;15(10):1164-75.

    Insert

    GFP (GFAP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLX304 Flag-mCherry-eEF1A1_IRES-GFP

    Plasmid

    #198383

    Purpose

    eEF1A1 fluorescent reporter

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    eEF1A1 (EEF1A1 Human)
    

    Use

    Lentiviral

    Tags

    Flag-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro

    Plasmid

    #176851

    Purpose

    lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    hYAP1-5SA (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S61A, S109A, S127A, S164A, S381A (phosphorylation…

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET Biotin His6 GFP LIC cloning vector (H6-msfGFP)

    Plasmid

    #29725

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, AviTag (biotin), SiriusGFP, and TEV cleava…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-MTS-jAspSnFR3-mRuby3

    Plasmid

    #203492

    Purpose

    Mitochondrial directed jAspSnFR3-mRuby3 in lentiviral expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    MTS-jAspSnFR3-mRuby3
    

    Use

    Lentiviral

    Tags

    His-tag, Mitochondrial localization signal, and m…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge