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168,035 results

Showing: 8481 - 8500 of 168035 results

  3. pD649-HAsp-NECTIN4-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156755
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    NECTIN4 (NECTIN4 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156755-plasmid-map-sequence-id-417859

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  9. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

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  11. TFORF2539

    Plasmid
    #143372
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX5 (SOX5 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143372-plasmid-map-sequence-id-400317

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  12. TFORF2542

    Plasmid
    #144128
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX5 (SOX5 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    144128-plasmid-map-sequence-id-396199

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  18. pGDP4 tet(A)

    Plasmid
    #112887
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    tet(A)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Sequence was codon optimized for E. coli K12 usin…
    Promoter
    Plac
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112887-plasmid-map-sequence-id-219894

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Showing: 8481 - 8500 of 168035 results