pBJ FLAG-hPar1
(Plasmid #53226)

• Purpose: mammalian expression of human Par1 with N-terminal FLAG tag
• Depositing Lab: Shaun Coughlin
• Publication: Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

Backbone

• Vector backbone: pBJ1
• Backbone manufacturer: Mark Davis lab
• Backbone size w/o insert (bp): 3300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Par1
• Alt name: coagulation factor II (thrombin) receptor
• Alt name: thrombin receptor
• Species: H. sapiens (human)
• Insert Size (bp): 3200
• Mutation: Deletion of aa1-39 (native signal peptide)
• Entrez Gene: F2R (a.k.a. CF2R, HTR, PAR-1, PAR1, TR)
• Promoter: SV40
• Tags / Fusion Proteins:
  • bovine prolactin signal sequence (MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS) (N terminal on insert)
  • FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: SV40pro F
• 3′ sequencing primer: EBV-rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The gene for hThrombin Receptor with Flag epitope at its amino terminus. The gene was cloned into the SalI site of pBluescript and excised and inserted into pBJ.