|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36972||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid #36953
- Backbone size (bp) 7052
Modifications to backboneeGFP replaced with mRFP
Vector typeMammalian Expression, Retroviral
- Promoter CMV
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer pLXSN_5 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Sugden lab plasmid p3313
Addgene's sequencing results differ from the full plasmid sequence provided by the depositing by a single nucleotide insertion between bp# 2412&2413 and a four nucleotide deletion of bp #2493-2496. These differences are in IRES and the depositing laboratory verified the IRES is functional.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCMMP-MCS-IRES-mRFP was a gift from Bill Sugden (Addgene plasmid # 36972 ; http://n2t.net/addgene:36972 ; RRID:Addgene_36972)
For your References section:The Latent Membrane Protein 1 (LMP1) Oncogene of Epstein-Barr Virus Can Simultaneously Induce and Inhibit Apoptosis in B Cells. Pratt ZL, Zhang J, Sugden B. J Virol. 2012 Apr;86(8):4380-93. Epub 2012 Feb 8. 10.1128/JVI.06966-11 PubMed 22318153