Purposeexpressed 7+ sortase A variant in E.coli
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||105602||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5500
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameS.aureus Sortase A, 7+ mutant
Insert Size (bp)500
- Promoter T7
/ Fusion Protein
- His tag (C terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET30b-7+-SrtA was a gift from Stephanie Dougan (Addgene plasmid # 105602 ; http://n2t.net/addgene:105602 ; RRID:Addgene_105602)
For your References section:Generation of Ca2+-independent sortase A mutants with enhanced activity for protein and cell surface labeling. Jeong HJ, Abhiraman GC, Story CM, Ingram JR, Dougan SK. PLoS One. 2017 Dec 4;12(12):e0189068. doi: 10.1371/journal.pone.0189068. eCollection 2017. PONE-D-17-29293 [pii] PubMed 29200433