164,718 results

1. pAAV-CAG-mNeonGreen

   Plasmid

   #99134

   Purpose

   An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the CAG promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   mNeonGreen
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-RLuc8.6-535

   Plasmid

   #87125

   Purpose

   Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

   Depositor

   Sanjiv Sam Gambhir

   Article

   Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

   Insert

   Renilla Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3. Flag-CSL WT

   Plasmid

   #167004

   Purpose

   Mammalian expression of CSL WT

   Depositor

   Gian-Paolo Dotto

   Article

   Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

   Insert

   CSL (RBPJ Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRISPR-SB

   Plasmid

   #177936

   Purpose

   Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

   Depositor

   Roland Rad

   Article

   Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

   Type

   Empty backbone

   Use

   CRISPR; Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Clover

   Plasmid

   #40259

   Purpose

   GFP with robust performance in protein fusions and useful as FRET donor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Has Service

   Cloning Grade DNA

   Insert

   Clover GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available Since

   Sept. 24, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. His SUMO HNRNPA1 dHexa

   Plasmid

   #197013

   Purpose

   E. coli expression vector

   Depositor

   Tanja Mittag

   Article

   Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

   Insert

   HNRNPA1
   

   Use

   Tags

   6His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AmCyan-P2A-mCherry

   Plasmid

   #45350

   Depositor

   Ilpo Huhtaniemi

   Article

   Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

   Insert

   AmCyan
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   SV40 NLS

   Promoter

   CMV

   Available Since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1a. pETM11-SUMO-SNCA-GFP

   Plasmid

   #107292

   Purpose

   Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility

   Depositor

   Dmytro Yushchenko

   Article

   Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191

   Insert

   SNCA-GFP (SNCA Human)
   

   Use

   Tags

   His-tag and SUMO-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF1a-IRES-Neo

   Plasmid

   #28019

   Depositor

   Thomas Zwaka

   Article

   Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINK-OTUD6B (full-length, aa 1-293)

    Plasmid

    #61418

    Purpose

    Expresses human OTUD6B (full-length) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA Flag ERbeta

    Plasmid

    #35562

    Depositor

    Harish Srinivas

    Article

    Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

    Insert

    Estrogen Receptor beta (ESR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2 TDP-43 NLS1, 5F-L YFP

    Plasmid

    #84913

    Purpose

    Mammalian expresion of TDP-43 NLS1, 5F-L YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKI1.1R

    Plasmid

    #85808

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    AtRPS5A

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-DIO-rM3D(Gs)-mCherry

    Plasmid

    #50458

    Purpose

    Double floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV8

    Insert

    rM3D-mCherry
    

    Use

    AAV

    Tags

    HA and mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDRF1-GW yoeGFP-T2A-mCherry

    Plasmid

    #118446

    Purpose

    Produces equimolar expression of yeast codon-optimized eGFP and mCherry

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yoeGFP-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-EF1adCas9VP64T2ABsd-W

    Plasmid

    #112919

    Purpose

    Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    dCas9VP64-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a promoter

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. TDP-REGv2(mScarlet-FLAG)#10 in pTwist-CMV backbone

    Plasmid

    #216154

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. Leaky but very sensitive to mild TDP-43 loss of function. It uses a cryptic exon. [Code 'B5'] Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic exon and C-terminal FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

    Plasmid

    #232879

    Purpose

    Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    5-Hydroxytryptamine Receptor 6 (HTR6 Human)
    

    Use

    Lentiviral

    Tags

    RGECO1.1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. amilCP chromoprotein

    Plasmid

    #117847

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, amilCP
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. UnReg-H2B-YFP-DD

    Plasmid

    #96892

    Purpose

    Reporter to monitor translation of unstructured mRNA

    Depositor

    Tobias Meyer

    Article

    Han et al Nat Methods. 2014 Jan;11(1):86-93. doi:

    Insert

    UnReg-H2B-Venus-DD
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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