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pEGFP-N1_MeCP2(WT)

Plasmid

#110186

Purpose

Expression of mouse MeCP2 (WT) in mammalian cells

Depositor

Adrian Bird

Article

Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

Insert

MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pZLCv2-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106357

Purpose

Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

Depositor

Stephen Tapscott

Article

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3XFLAG and HA

Expression

Mammalian

Promoter

U6

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-CA

Plasmid

#105665

Purpose

expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Tags

6x his tag

Expression

Bacterial

Promoter

pBad/arabinose

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pET30b-7+-SrtA

Plasmid

#105602

Purpose

expressed 7+ sortase A variant in E.coli

Depositor

Stephanie Dougan

Article

Jeong et al PLoS One. 2017 Dec 4;12(12):e0189068. do

Insert

S.aureus Sortase A, 7+ mutant

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti_CamKIIa_GFP

Plasmid

#96941

Purpose

To label mature excitatory iPSC-derived human neurons

Depositor

Ricardo Dolmetsch, Oleksandr Shcheglovitov

Article

Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

Insert

Green fluorescent protein

Use

Lentiviral

Promoter

CaMKIIa

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSAM_Cam

Plasmid

#91570

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

Depositor

Harry Mobley

Article

Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only
pLV hUbC-dCas9-MQ1(Q147L)-EGFP

Plasmid

#89793

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR and Lentiviral

Tags

3*FLAG and T2A-EGFP

Expression

Mammalian

Mutation

Q147L, CAA-->CTA

Promoter

hUbC

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1X-Nup93

Plasmid

#87336

Purpose

To express human Nup93 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH Yap S127A

Plasmid

#86497

Purpose

Mammalian expression of FLAG-tagged Yap containing S127A mutation

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes associated protein 1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-C1

Plasmid

#84355

Purpose

Expresses mCyRFP1 fluorescent protein in an empty backbone

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1

Expression

Mammalian

Mutation

A161K and N41A, reletive to CyRFP1

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N

Plasmid

#80930

Purpose

Expresses Cas9N in mammalian cells.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pJMP2

Plasmid

#79874

Purpose

Bacillus subtilis sgRNA expression vector; integrates into amyE

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFUSE2ss-CLIg-mK_M18

Plasmid

#82358

Purpose

Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

Insert

mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)

Tags

none

Expression

Mammalian

Promoter

hEF1-HTLV prom

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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LeGO-lnc

Plasmid

#80624

Purpose

Lentiviral lncRNA Overexpression

Depositor

Jan-Henning Klusmann

Article

Emmrich et al Mol Cancer. 2014 Jul 15;13:171. doi: 10.

Insert

empty control

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001149198)

Plasmid

#80248

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pJJR82

Plasmid

#75027

Purpose

EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Mike Boxem

Article

Ramalho et al Development. 2020 Jul 22;147(14):dev1880

Insert

EGFP^SEC^3xFlag

Use

CRISPR

Tags

3xFLAG and C. elegans codon optimized EGFP

Expression

Worm

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMOS003-lenti-CMV-GCaMPer

Plasmid

#65227

Purpose

ER calcium sensor for lentivirus production

Depositor

Adam Cohen, Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

GCaMPer

Use

Lentiviral

Tags

circularly permuted GFP

Expression

Mammalian

Mutation

GCaMP3 (D324G, D360G, D397G, D435G)

Promoter

CMV

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-Luc2-P2A-copGFP

Plasmid

#72485

Purpose

Express Luc2 and copGFP

Depositor

Kazuhiro Oka

Insert

Luc2-P2A-copGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1

Available Since

Jan. 11, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C3-PLK4-3xFLAG

Plasmid

#69837

Purpose

This plasmid encodes PLK4 isoform 1 with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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