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173,632 results

Showing: 8401 - 8420 of 173632 results

  4. TRUPATH Triple Gai1

    Plasmid
    #196048
    Purpose
    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196048-plasmid-map-sequence-id-385883

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  7. pL6puro-pSV40(1)-pmRevErbA-Venus-NLS-Pest

    Plasmid
    #240112
    Purpose
    A lentiviral (yellow) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.
    Depositor
    Achim Kramer
    Article
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+Venus-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added SV40-Enhancer
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240112-plasmid-map-sequence-id-481768

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  8. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  13. p41Nat MagicFluor

    Plasmid
    #58563
    Purpose
    p41Nat carrying both mating type markers buffered by the Cyc1 terminator
    Depositor
    Leonid Kruglyak
    Insert
    Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
    Expression
    Yeast
    Available Since
    Nov. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    58563-plasmid-map-sequence-id-90668

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Showing: 8401 - 8420 of 173632 results