166,289 results

1. LsgRNA-MS2

   Plasmid

   #235597

   Purpose

   sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF3008

   Plasmid

   #144484

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CDX2 (CDX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3

   Plasmid

   #108912

   Purpose

   Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Has Service

   AAV9

   Insert

   ChroME-ST
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3β-FLAG-CBP-HA

   Plasmid

   #32908

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   CBP (Crebbp Mouse)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. hAAVS1 1L TALEN

   Plasmid

   #35431

   Purpose

   TALEN designed to target the human AAVS1 locus

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   hAAVS1 1L TALEN
   

   Expression

   Mammalian

   Available Since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pScaffold-H1 donor

   Plasmid

   #118152

   Purpose

   PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a.Tat(48-57)-EGFP

   Plasmid

   #165040

   Purpose

   Expression of Tat(48-57) conjugated EGFP for protein purification (6XHis tag)

   Depositor

   Dokyun Na

   Article

   Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

   Insert

   EGFP
   

   Tags

   6XHis and Tat(48-57)

   Expression

   Bacterial

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF0096

   Plasmid

   #142459

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAK184

   Plasmid

   #167281

   Purpose

   MazF-based mutagenesis vector for C. difficile

   Depositor

   Robert Fagan

   Article

   Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

   Type

   Empty backbone

   Use

   E. coli - c. difficile shuttle vector

   Promoter

   Pxyl

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. MO91-dCas9-AID(P182X)

    Plasmid

    #83260

    Purpose

    Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

    Depositor

    Xing Chang

    Article

    Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

    Insert

    dCas9-AID P182X
    

    Use

    Retroviral

    Tags

    3xFLAG-NLS and FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9; P182X in AID

    Promoter

    MSCV

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

11. 12xMoonTag-12xSunTag-kif18b-24xPP7

    Plasmid

    #128606

    Purpose

    Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    12xMoonTag-12xSunTag
    kinesin family member 18b (KIF18B Human)
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TMPRSS2

    Plasmid

    #53887

    Purpose

    Expression of TMPRSS2

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Has Service

    Cloning Grade DNA

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pHAGE-PDGFRB

    Plasmid

    #116770

    Purpose

    Lentiviral expression of PDGFRB

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. MAC_C_EPHB2

    Plasmid

    #187797

    Purpose

    MAC-tagged gene expression of human EPHB2

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    EPHB2 (EPHB2 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmNeonGreenHO-G

    Plasmid

    #127912

    Purpose

    a mammalian expression backbone vector for humanized mNeonGreen

    Depositor

    Isei Tanida

    Article

    Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

    Insert

    humanized mNeonGreen
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LeGO-V2

    Plasmid

    #27340

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, Venus fluorescent protein
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIRE4-Bpa

    Plasmid

    #155342

    Purpose

    Plasmid encoding the translational pair that enables efficient incorporation of Benzoyl-phenylalanine in response to the amber codon into proteins in mammmalian cells.

    Depositor

    Irene Coin

    Article

    Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

    Insert

    humanized EBpaRS/BstYam
    

    Expression

    Mammalian

    Promoter

    PGK/U6

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOpen-T4 PNK

    Plasmid

    #165520

    Purpose

    The most commonly used polynucleotide kinase.

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    T4 PNK
    

    Use

    Synthetic Biology

    Available Since

    Aug. 3, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. mTurquoise2

    Plasmid

    #198196

    Purpose

    Spectral correction: Expression of mTurquoise2

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    mTurquoise2
    

    Expression

    Mammalian

    Mutation

    A206K

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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