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169,724 results

Showing: 8401 - 8420 of 169724 results

  7. pOpen-Pwopol

    Plasmid
    #165562
    Purpose
    DNA polymerase; additionally exhibits 3' to 5' exonuclease activity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Pwo DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165562-plasmid-map-sequence-id-340035

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  12. pEXPqcxip-hFTSJ3-FLAG

    Plasmid
    #165463
    Purpose
    Human FTSJ3 retroviral mammalian expression vector. FLAG/Gateway modified pQCXIP vector with C-terminal FLAG epitope and bicistronic puromycin expression.
    Depositor
    Martin Dorf
    Article
    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11
    Insert
    FtsJ RNA 2'-O-methyltransferase 3 (FTSJ3 Human)
    Use
    Retroviral; Gateway
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV/MSV, 5'LTR
    Available Since
    March 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    165463-plasmid-map-sequence-id-327804

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  13. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

    Plasmid
    #103865
    Purpose
    Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    PspCas13b
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    Contains the H133A and H1058A mutations which ina…
    Promoter
    EF1a
    Available Since
    Jan. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    103865-plasmid-map-sequence-id-243540

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  15. TFORF2399

    Plasmid
    #144334
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZFPM1 (ZFPM1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    144334-plasmid-map-sequence-id-427297

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  18. pOpen-K12polLF

    Plasmid
    #165511
    Purpose
    DNA pol fragment that retains 5'-3' polymerase activity and 3'-5' exonuclease activity but loses 5'-3' exonuclease activity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Polymerase I, Large (Klenow) Fragment
    Use
    Synthetic Biology
    Available Since
    July 30, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165511-plasmid-map-sequence-id-338596

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Showing: 8401 - 8420 of 169724 results