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pCAGEN

Plasmid

#11160

Purpose

Mammalian expression vector for cloning and expressing your gene under the CAG promoter

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

modified multiple cloning sites

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI WT

Plasmid

#207832

Purpose

telomere specific nuclease to create double-strand breaks at telomeres

Depositor

Roger Greenberg

Article

Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

Flag, DD, ER, mCherry and FokI

Expression

Mammalian

Promoter

TRE3G

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV BiP-Myc-KDEL-wt

Plasmid

#27164

Purpose

Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Tags

KDEL and Myc

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCE-GFP

Plasmid

#41858

Purpose

Non-integrating (episomal) expression of EGFP

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pMCP-RecT

Plasmid

#183827

Purpose

pLenti-EF1A-MCP-EXTEN-RecT-NLS

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

MCP-RecT_NLS

Expression

Mammalian

Promoter

EF1A

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pHelper_TS_V2_ampR

Plasmid

#214467

Purpose

Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.

Depositor

Scott Saunders

Article

Saunders et al bioRxiv 2023

Insert

CspRecT / Bxb-1

Use

Synthetic Biology

Promoter

XylS / AraC

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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AiP2105 - pAAV-AiE2638m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#230551

Purpose

AiE2638m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Cherry-Fibrillarin-C1

Plasmid

#134539

Purpose

Expresses Cherry-Fibrillarin fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Fibrillarin (FBL Human)

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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psfGFP-LAMP1-mCherry (pHLARE)

Plasmid

#164477

Purpose

Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry.

Depositor

Diane Barber

Article

Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

Insert

LAMP1 (Lamp1 Rat)

Tags

mCherry and superfolder GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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LTR5HS S. pyogenes scaffold CARGO array

Plasmid

#191316

Purpose

Contains an array of guide RNAs targeting LTR5Hs elements

Depositor

Joanna Wysocka

Article

Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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Nme2Cas9_AAV

Plasmid

#119924

Purpose

All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Use

AAV

Tags

NLS-3xHA-NLS and NLS-NLS

Expression

Mammalian

Promoter

U1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE8e

Plasmid

#181751

Purpose

MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE8e

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-H_I3

Plasmid

#239343

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mScarlet3-H (a.k.a. mYongHong)

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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synIRES_RC25

Plasmid

#189970

Purpose

Part 2 plasmid to introduce the HRV-B3 IRES with proximal loop Apt-eIF4G into circRNAs

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

synIRES_RC25

Use

Cloning

Promoter

N/A

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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ABE8.20-SpRY

Plasmid

#226859

Purpose

Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ABE8.20-SpRY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen

Plasmid

#182387

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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HTR2A-Tango

Plasmid

#66409

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

HTR2A (HTR2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pDD427

Plasmid

#202080

Purpose

Frame +2/-1 selector for NHEJ knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

pEF1a-mCherry-Cas9 + Frame +2/-1 sgRNA

Use

CRISPR

Promoter

EF1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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[#GS24 -LV] Syn1-GFP-OMP25

Plasmid

#233566

Purpose

Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

eGFP (eGFP Synthetic, Aequorea victoria)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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Tubulin-mGreenLantern

Plasmid

#164460

Purpose

Localization of mGreenLantern fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Tubulin

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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