164,649 results

1. pTRIP-SFFV-EGFP-NLS

   Plasmid

   #86677

   Purpose

   Expresses nuclear-localized GFP after lentiviral transduction. Can be used to monitor GFP leakage into the cytosol following nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   EGFP-NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bet1-mCherry

   Plasmid

   #209848

   Purpose

   Expresses Bet1-mCherry in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   Bet1 (BET1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EB1-tdTomato

   Plasmid

   #50825

   Purpose

   expresses EB1-tdTomato fusion in mammalian cells for labeling the growing tips of MTs

   Depositor

   Erik Dent

   Insert

   EB1 (Mapre1 Mouse)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJC5-3XMyc-EGFP-PEX26

   Plasmid

   #139059

   Purpose

   Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

   Depositor

   David Sabatini

   Article

   Ray et al iScience. 2020 May 22;23(5):101109. doi:

   Insert

   3XMyc-EGFP-PEX26
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   UBC

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Sec61β

   Plasmid

   #172445

   Purpose

   Expression of ER protein Sec61beta with an N-terminal mCherry tag

   Depositor

   Stephen Royle

   Article

   Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   Protein transport protein Sec61 subunit beta
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROP_C9_Puro_ERBB2

   Plasmid

   #183287

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   ERBB2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB064d

   Plasmid

   #79197

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Plac62 luxAB; Ppro1 434cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac62; Ppro1

   Available Since

   Dec. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH6HTC_FAM98A-Halo

   Plasmid

   #175329

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   FAM98A (FAM98A Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-HIFR

   Plasmid

   #192946

   Purpose

   Lentiviral production of HIF-1alpha reporter

   Depositor

   Markus Covert

   Article

   Jeknic et al J Biol Chem. 2023 Mar 10:104599. doi: 10

   Insert

   HIF-1alpha reporter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mCMV

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRESneo-FLAG/HA Ago2 corrected

    Plasmid

    #10822

    Depositor

    Thomas Tuschl

    Article

    Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    3 silent mutations in orf

    Promoter

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPGK-T7/2-CD44s (-cyt)

    Plasmid

    #137815

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44s (-cyt) (CD44 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIG-831_HA-GD2-28z_CAR_TFAP4_Retroviral

    Plasmid

    #207508

    Purpose

    This plasmid can be used to generate retrovirus.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSMOX-mNG::LacI-V3

    Plasmid

    #210224

    Purpose

    Integrates a T7 RNA polymerase, tetracycline repressor and an mNeongreen Lac repressor fusion protein with a puromycin drug selection marker into the tubulin locus.

    Depositor

    Calvin Tiengwe

    Article

    Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

    Insert

    mNeongreen Lac repressor fusion protein
    

    Use

    Trypanosoma brucei expression

    Tags

    Expression

    Mutation

    Promoter

    Pol II readthrough transcription after integration

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. SB52

    Plasmid

    #21487

    Depositor

    Shoumo Bhattacharya

    Article

    Bhattacharya et al Genes Dev. 1999 Jan 1. 13(1):64-75.

    Insert

    Cited2 (CITED2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 23, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pRMC2

    Plasmid

    #68940

    Purpose

    tetracycline-inducible expression vector for Staphylococcus aureus

    Depositor

    Tim Foster

    Article

    Corrigan et al Plasmid. 2009 Mar;61(2):126-9. doi: 10.1

    Type

    Empty backbone

    Use

    Tet inducible e. coli/staphylococcus shuttle vect…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. nLuc_AP4_TEVs_P3mS

    Plasmid

    #119299

    Purpose

    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nLuc fused to antiparallel Coiled-coil AP4 connected with P3 via TEV cleavable linker
    

    Use

    Luciferase

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. opto-RhoA-mCherry_pcDNA3.1

    Plasmid

    #164472

    Purpose

    Expresses opto-RhoA (RhoA-GGGSx2-BcLOV4-GGGSx2-mCherry) in a pcDNA3.1 backbone.

    Depositor

    Brian Chow

    Article

    Berlew et al Adv Biol. 2021 Sep;5(9):e2100810. doi: 1

    Insert

    opto-RhoA
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHN922

    Plasmid

    #178975

    Purpose

    Expression of GST-His8-GFP-nanobody

    Depositor

    Kazuhiro Abe

    Insert

    GST-His8-GFP-nanobody
    

    Use

    Affinity Reagent/ Antibody

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29b-H6_Streptavidin_sfGFP

    Plasmid

    #124296

    Purpose

    His6-tagged Streptavidin GFP fusion protein

    Depositor

    Mark Arbing

    Insert

    His6 tag - TEV protease site - T7 tag - Streptavidin (15-159) - superfolder GFP
    

    Use

    Tags

    His6 and T7 tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAd5-B1-deltaE1-GFP

    Plasmid

    #179203

    Purpose

    Block 1 with deletion in E1 and insertion of GFP expression cassette

    Depositor

    Fred Bunz

    Article

    Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

    Insert

    Modified Block 1 with deletion in E1 and insertion of GFP expression cassette
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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