162,309 results

1. pBA1015

   Plasmid

   #189589

   Purpose

   T7gp1.7 deletion HR vector 250bp HR Arms (T7 phage editing)

   Depositor

   Jennifer Doudna

   Article

   Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

   Insert

   T7gp1.7 Locus 250bp Homology Arms
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Encodes for a full deletion of T7gp1.7

   Promoter

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC18T-mini-Tn7T-Gm-Pc-sfGFP

   Plasmid

   #199248

   Purpose

   Fluorescent labelling of bacterial cells with sfGFP

   Depositor

   Uwe Mamat

   Article

   Mamat et al Appl Environ Microbiol. 2023 May 17:e003

   Insert

   Codon-optimized sfGFP gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimization for Xanthomonadaceae

   Promoter

   Pc promoter from class III integron of Delftia a…

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBPGUw

   Plasmid

   #17575

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Tools for neuroanatomy and neurogenetics

   Insert

   GAL4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPRv2_AMBRA1#2

   Plasmid

   #174153

   Purpose

   Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene

   Depositor

   Julien Sage

   Article

   Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

   Insert

   AMBRA (AMBRA1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138131

   Purpose

   Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mCherry-anti-rabbit-IgG(Fc)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBABEpuro-ERBB2

   Plasmid

   #40978

   Depositor

   Matthew Meyerson

   Article

   Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBJ FLAG-hPar1

   Plasmid

   #53226

   Purpose

   mammalian expression of human Par1 with N-terminal FLAG tag

   Depositor

   Shaun Coughlin

   Article

   Ishii et al J Biol Chem. 1993 May 5. 268(13):9780-6.

   Insert

   Par1 (F2R Human)
   

   Use

   Tags

   FLAG and bovine prolactin signal sequence (MDSKGS…

   Expression

   Mammalian

   Mutation

   Deletion of aa1-39 (native signal peptide)

   Promoter

   SV40

   Available since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pL0M-S-mTurquoise2-EC18152

   Plasmid

   #137074

   Purpose

   Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

   Depositor

   Julian Hibberd

   Article

   Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

   Insert

   mTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMV Blast empty (w263-1)

   Plasmid

   #17486

   Purpose

   3rd gen lentiviral empty control vector, CMV promoter, Blast 

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAG:: mCherry-2a-hM4Dnrxn

    Plasmid

    #52523

    Purpose

    Co-expresses hM4Dnrxn and a red fluorescent label

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    mCherry-2a-hM4Dnrxn
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CAg

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. EFSp-GFP-Empty

    Plasmid

    #174171

    Purpose

    Control lentiviral vector expressing GFP under control of the EFS promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pC0078 RESCUE

    Plasmid

    #130661

    Purpose

    pCMV-dCas13b6-mapkNES-GS-dADAR2(E488Q/V351G/S486A/T375S/S370C/P462A/N597I/L332I/I398V/K350I/M383L/D619G/S582T/V440I/S495N/K418E/

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

    Insert

    dRanCas13b-ADAR2dd (RESCUE)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. N1-Tq-Ca-FLITS

    Plasmid

    #191454

    Purpose

    Genetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changes

    Depositor

    Dorus Gadella

    Article

    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:

    Insert

    Tq-Ca-FLITS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe puro c-met WT

    Plasmid

    #17493

    Depositor

    Joan Brugge

    Article

    Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

    Insert

    c-Met (Met Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmEGFP-C1

    Plasmid

    #36412

    Depositor

    Benjamin Glick

    Type

    Empty backbone

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVX-EF1alpha-SARS-CoV-2-nsp6-2xStrep-IRES-Puro

    Plasmid

    #141372

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp6 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pBMCL1

    Plasmid

    #178203

    Purpose

    An improved vector for BacMam system

    Depositor

    Lei Chen

    Article

    Guo et al Biochem Biophys Res Commun. 2022 Feb 26;

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-SF2

    Plasmid

    #17990

    Depositor

    Tom Misteli

    Article

    Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains SF2 (aa1-210)

    Promoter

    Available since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-DN-hCUL4A-FLAG

    Plasmid

    #15821

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin4A (CUL4A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N440

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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