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We narrowed to 62,186 results for: cloning

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Showing: 8261 - 8280 of 62186 results

  1. pHBJT005

    Plasmid
    #225156
    Purpose
    Low copy cloning vector for integration of C-terminal FLAG epitope tag (ChlR). SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    FLAG epitope tag (35 bp)
    Expression
    Bacterial
    Available Since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225156-plasmid-map-sequence-id-444672

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  2. pHBJT004

    Plasmid
    #225155
    Purpose
    Medium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    FLAG epitope tag (35 bp)
    Expression
    Bacterial
    Available Since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225155-plasmid-map-sequence-id-444671

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  3. pHBJT003

    Plasmid
    #225154
    Purpose
    Medium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    FLAG epitope tag (35 bp)
    Expression
    Bacterial
    Available Since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225154-plasmid-map-sequence-id-444670

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  4. pHBJT002

    Plasmid
    #225153
    Purpose
    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    FLAG epitope tag (35 bp)
    Expression
    Bacterial
    Available Since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225153-plasmid-map-sequence-id-443288

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  16. pHmK-C1

    Plasmid
    #87866
    Purpose
    Backbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1
    Depositor
    Isei Tanida
    Article
    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi
    Insert
    pHluorin-mKate2 fusion
    Tags
    pHluorin-mKate2 fusion
    Expression
    Mammalian
    Available Since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    87866-plasmid-map-sequence-id-252335

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  17. pRVL-3

    Plasmid
    #104581
    Purpose
    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and E318A/K320A/K322A
    Promoter
    CMV
    Available Since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    104581-plasmid-map-sequence-id-201392

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  18. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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Showing: 8261 - 8280 of 62186 results