Addgene

Showing: 821 - 840 of 4047 results

Results per page:

Sort results by:

C455-E01: attB4-stuffer-attB5

Plasmid

#162983

Purpose

Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C515-E01: attB5r-stuffer-attB1r

Plasmid

#162968

Purpose

Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

stuffer fragment for vector controls

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SRS-MTBD(26/19) AB(monomer), wt

Plasmid

#22381

Depositor

Ron Vale

Article

Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-WingGFP-tan

Plasmid

#83473

Purpose

EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

Depositor

Patricia Wittkopp

Article

Lamb et al Fly (Austin). 2016 Aug 5:1-12.

Insert

WingGFP-tan

Use

CRISPR

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Hsp70 and enhancer from D. melanogaster yellow ge…

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r

Plasmid

#162958

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C234-E08: attB2r-3xFLAG-attB3

Plasmid

#162912

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

triple FLAG epitope tag

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C514-E44: attB5r-SNAP-nostop-attB1r

Plasmid

#162962

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

SNAP tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r

Plasmid

#162967

Purpose

Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Dual Strep2 purification tag with upstream HTLV RU5 5' UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH3577

Plasmid

#179612

Purpose

Pceh-12::Chrimson::Cherry unc-54 3' UTR C.elegans VB-MNs expression of Chrimson::Cherry

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

Chrimson

Use

Tags

Cherry

Expression

Worm

Mutation

Promoter

Pceh-12

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH3558

Plasmid

#179611

Purpose

Punc-4::Chrimson::Cherry unc-54 3' UTR C.elegans A-MNs and others expression of Chrimson::Cherry

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

Chrimson

Use

Tags

Cherry

Expression

Worm

Mutation

Promoter

Punc-4

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT/TO/intron/FlagCter

Plasmid

#113548

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

Flag

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
C239-E03: attB2r-IRES-ffLuc2-pA-attB3

Plasmid

#162919

Purpose

Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

firefly luciferase with upstream IRES sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAd388-shLuc

Plasmid

#83275

Purpose

shRNA targeting luciferace

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA to Luciferase

Use

Retroviral

Tags

Expression

Mutation

Promoter

pUC ori

Available since

Dec. 2, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLLOXmPOLBeta

Plasmid

#24174

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Jan 22. ():.

Insert

pLLOXmPOLbeta (Polb Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

x

Promoter

Available since

May 27, 2010

Availability

Academic Institutions and Nonprofits only
EcoHIV-mScarlet

Plasmid

#235598

Purpose

EcoHIV-mScarlet is a modification of pNL4-3-EcoHIV (Potash et al, PNAS 2005). EcoHIV is rodent-infectable modification of HIV where HIV env replaced for MLV-env

Depositor

Michael Shtutman

Article

Li et al J Vis Exp. 2024 Dec 20;(214). doi: 10.37

Insert

NL4-3 EcoHIV mScarlet

Use

Lentiviral

Tags

mScarlet

Expression

Mutation

Promoter

Available since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac SOX R139A

Plasmid

#192058

Purpose

Expression vector for SOX (ORF37) R139A

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37)

Use

Tags

Expression

Insect

Mutation

R139A

Promoter

Polyhedrin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pACE WT SOX pACEmam1

Plasmid

#192049

Purpose

Expresses WT SOX (pACEmam1)

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

WT SOX

Use

Unspecified

Tags

Expression

Mutation

Promoter

CMV

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac SOX Q129H

Plasmid

#192057

Purpose

Expression vector for SOX (ORF37) Q129H

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37) Q129H

Use

Tags

Expression

Insect

Mutation

Q129H

Promoter

Polyhedrin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57 mini-ZsGreen-P2A

Plasmid

#222648

Purpose

Vector for cloning gene of interest for in vitro transcription

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

ZsGreen

Use

In vitro transcription

Tags

Expression

Mutation

mutated stop codon prior to a P2A peptide

Promoter

T7

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pNK875

Plasmid

#219735

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtTCTP

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter from Arabidopsis translationally controlled tumor protein (AtTCTP) gene

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

AtTCTP

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge