Addgene

We narrowed to 2,601 results for: GUS

Showing: 821 - 840 of 2601 results

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pSLQ10844

Plasmid

#183956

Purpose

CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, Hygromycin Resistance, and mCherry

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Promoter

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL8

Plasmid

#149519

Purpose

Express APEX2-Galectin-8 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS8 (LGALS8 Human)

Use

Tags

APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOf

Plasmid

#189932

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOf

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL3

Plasmid

#149518

Purpose

Express APEX2-Galectin-3 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS3 (LGALS3 Human)

Use

Tags

APEX2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESRRB_Hormone-recep

Plasmid

#109909

Purpose

Protein expression and purification of ESRRB_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESRRB_Hormone-recep (ESRRB Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(+)-NEMOb

Plasmid

#189934

Purpose

Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

Depositor

Yubin Zhou

Article

Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

Insert

NEMOb

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV:cTNT::Luciferase

Plasmid

#69915

Purpose

Used to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.

Depositor

William Pu

Article

Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

Insert

Firefly luciferase

Use

AAV

Tags

Expression

Mutation

Promoter

Chicken cardiac troponin T

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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FR_HNF4A8

Plasmid

#31114

Depositor

Gerhart Ryffel

Article

Erdmann et al Biol Chem. 2007 Jan . 388(1):91-106.

Insert

HNF4A (HNF4A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

splice variant 8

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pEF1a-BirA-V5-neo

Plasmid

#100548

Purpose

Expresses C-terminal V5-tagged BirA biotin ligase

Depositor

Jian Xu

Article

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Insert

C-terminal V5-tagged BirA

Use

Tags

V5, His

Expression

Mammalian

Mutation

Promoter

Human EF1a

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ10875

Plasmid

#183963

Purpose

CBA-hyperdCas12a-HA-KRAB-mCherry

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, KRAB, and mCherry

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CBA

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ1651-sgRNA(F+E)-sgGal4

Plasmid

#100549

Purpose

sgGal4-expressing vector modified from Addgene plasmid #51024

Depositor

Jian Xu

Article

Liu et al Cell. 2017 Aug 24;170(5):1028-1043.e19.

Insert

sgGal4

Use

CRISPR

Tags

sgRNA

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1amCherry-2A-OsTIR1-W

Plasmid

#159296

Purpose

Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

Depositor

Kosuke Yusa

Article

Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

Insert

OsTIR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human EF1a promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pNICKclos1.0

Plasmid

#73639

Purpose

Genome editing for gene pyrE (CAC-002) in Clostridium acetobutylicum ATCC 824

Depositor

Sheng Yang

Article

Li et al Biotechnol J. 2016 May 23. doi: 10.1002/

Inserts

Cas9 nickase
sGRNA to pyrE

Use

E.coli-clostridium shuttle vector

Tags

Expression

Mutation

D10A

Promoter

j23119 and ptb

Available since

June 17, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-Foxo1-ADA 6KR

Plasmid

#17561

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1-ADA 6KR (Foxo1 Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-Foxo1-ADA 6KQ

Plasmid

#17563

Depositor

Domenico Accili

Article

Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

Insert

Foxo1 ADA 6KQ (Foxo1 Mouse)

Use

Tags

Flag and Myc

Expression

Mammalian

Mutation

K242, K245, K259, K262, K271 and K291 were replac…

Promoter

CMV

Available since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only
pHH0103_ACTG2_Actin_2

Plasmid

#109990

Purpose

Protein expression and purification of ACTG2_Actin_2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ACTG2_Actin_2 (ACTG2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAI3_G-alpha

Plasmid

#110014

Purpose

Protein expression and purification of GNAI3_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAI3_G-alpha (GNAI3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ133-RZ-Cas12j2

Plasmid

#189784

Purpose

Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Article

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ10704

Plasmid

#183957

Purpose

U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

SKSKSO array

Use

Lentiviral

Tags

EF1a-Puro-T2A-TagBFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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