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pLKO.1 puro_shTBK1_3185

Plasmid

#167294

Purpose

Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

shRNA TBK1 (TBK1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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opto-Rac1

Plasmid

#135396

Purpose

Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.

Depositor

Brian Chow

Article

Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3

Insert

opto-Rac1

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pGL2- Empty control -SV40-Luc

Plasmid

#26280

Purpose

Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pDULE-ABK

Plasmid

#49086

Purpose

A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

Depositor

Peter Schultz

Article

Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

Inserts

tRNA synthetase
tRNA for a photocrosslinking amino acid

Expression

Bacterial

Mutation

L274M, C313A, Y349F

Promoter

GlnRS and lpp

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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human oct4-GFP

Plasmid

#21153

Depositor

Rudolf Jaenisch

Article

Green et al Nano Lett. 2008 Oct . 8(10):3126-30.

Insert

hOCT4-GFP (POU5F1 Human)

Use

Human targeting

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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mCherry-p62-Silent_Mutation_WT

Plasmid

#187986

Purpose

human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Tags

mCherry

Expression

Mammalian

Mutation

silent mutation for resistance to sip62 #5

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRαγεζ

Plasmid

#187351

Purpose

Lentiviral expression of GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR alpha, and truncated hCD3 (gamma, epsilon, and zeta)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-HO-G

Plasmid

#164163

Purpose

Expresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminal

Depositor

Isei Tanida

Article

Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

Insert

mEGFP A236K

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINB-AMSH*

Plasmid

#66712

Purpose

Expresses activated AMSH in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

hbp (Stam2 Mouse)
AMSH (STAMBP Human)

Tags

His-3C

Expression

Bacterial

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pOPINK-TRABID (NZF1-3, aa 1-178)

Plasmid

#66185

Purpose

Expresses TRABID NZF1-3 (aa 1-178) in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

TRABID (ZRANB1 Human)

Tags

His6-GST

Expression

Bacterial

Mutation

only codes for amino acids 1-178 of TRABID

Promoter

T7

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-c-Myc-T58A

Plasmid

#13372

Purpose

Retroviral expression of mouse c-Myc T58A mutant

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

myelocytomatosis oncogene (T58A mutant) (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

Tyrosine 58 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-MitoRlucN-T2A-RlucCER-IRES-mCherry

Plasmid

#249563

Purpose

For detection of ER-mitochondrial contact

Depositor

Ginam Cho, Jeffrey Golden, Youngshin Lim

Article

Chen et al J Vis Exp. 2024 Oct 11;(212):10.3791/668

Insert

MitoRlucN-T2A-RlucCER

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Promoter

Chicken beta-actin promoter with CMV enhancer

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pL0_CD-greenlantern

Plasmid

#202141

Purpose

Level 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Green lantern gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pHR-SFFV_3C-Twin-Strep

Plasmid

#113900

Purpose

Mammalian protein expression

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

SFFV

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-mitoT

Plasmid

#174519

Purpose

Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells

Depositor

Oleh Khalimonchuk

Article

Viana et al iScience. 2021 Jan 29;24(2):102119. doi:

Insert

MitoT synthetic tether

Tags

6xHis and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pTK88_GFP-Anillin

Plasmid

#46354

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

Anillin (ANLN Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

a1854 is changed to c to disrupt XhoI site withou…

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pP19 (GB0038)

Plasmid

#68195

Purpose

Provides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

P19 coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk3a2

Plasmid

#13759

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2 (MAPK10 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA5_FRT_TO_TurboID-mCherry-Cry2

Plasmid

#166504

Purpose

Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.

Depositor

Angelos Constantinou

Article

Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2

Insert

None

Tags

TurboID and mCherry-CRY2

Expression

Mammalian

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBXNPHM3

Plasmid

#110099

Purpose

E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage site

Depositor

Markus Seeger

Article

Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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