We narrowed to 10,605 results for: yeast

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMW#3

Plasmid

#13350

Depositor

Marian Walhout

Article

Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

Insert

LacZ reporter

Expression

Yeast

Available Since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

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pSCARS1

Plasmid

#174868

Purpose

Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

Depositor

Zengyi Shao

Article

Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

Insert

ARS1

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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BiFC3

Plasmid

#190048

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Expression

Yeast

Promoter

Met3

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC4

Plasmid

#190049

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 173–238)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (ATG-aa173-238)

Expression

Yeast

Promoter

Met3

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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BiFC2

Plasmid

#190047

Purpose

BiFC plasmid for C-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172 - TAA)

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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BiFC1

Plasmid

#190046

Purpose

BiFC plasmid for N-terminal tagging of genes with yEmVenus (aa 1–172)

Depositor

Patrick Van Dijck

Article

Subotic et al G3 (Bethesda). 2017 Oct 5;7(10):3509-352

Insert

YemVenus (aa1-172)

Expression

Yeast

Promoter

Met3

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pGY53

Plasmid

#221709

Purpose

Empty Cas12a vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pL-rpb1-N488D

Plasmid

#10988

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-N488D (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 1462 A to G in the RPB1 ORF resulting in…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pL-rpb1-E1103G

Plasmid

#10989

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

rpb1-E1103G (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Mutation 3308 A to G in the RPB1 ORF resulting in…

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only
pPMVAu8

Plasmid

#98297

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

PRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCT-tRNA

Plasmid

#133813

Purpose

CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

Depositor

Geraldine Butler

Article

Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

Insert

cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter

Use

CRISPR

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BYG

Plasmid

#158126

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pFTK093

Plasmid

#171365

Purpose

Vector part (LVL1) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

sgRNA transcription unit (MoClo lvl1 unit), P-gpdA-HH-sgRNA-HDV-Ttrpc, replacable LacZ gene

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pWS2247

Plasmid

#123026

Purpose

Tet promoter (TetO(x7)-pLEU2m)

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

pTet [2]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDUP3

Plasmid

#92174

Purpose

Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

Depositor

Judith Berman

Article

Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

Insert

NAT1

Use

Genbank: u03440.1

Expression

Yeast

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pRG646_lexO-HO_LexA-TF_LEU2MX

Plasmid

#154815

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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eGFP L202 gRNA

Plasmid

#119132

Purpose

gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L202 gRNA

Use

CRISPR

Mutation

None

Promoter

U6

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pADH137

Plasmid

#90986

Purpose

NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Inserts

C. albicans LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2

Use

CRISPR

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDS244

Plasmid

#34982

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Ste11 (STE11 Budding Yeast)

Expression

Yeast

Promoter

ADH

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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