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pDTnLAPP2A frame 2

Plasmid

#28228

Depositor

Harald von Melchner

Article

Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

Insert

Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein

Use

Retroviral

Tags

EGFP and nLAP-tag

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pLM032

Plasmid

#134244

Purpose

Retroviral plasmid with H3.1A EGFP insert

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

Human H3.1A + eGFP/HALO retroviral tagging

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pME-6MTzactMap2k2b

Plasmid

#67710

Purpose

Middle entry clone

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

activated Map2k2b (map2k2b Zebrafish)

Mutation

S221D, S225D

Promoter

none

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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Δconst-GFP

Plasmid

#33114

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γA3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of the C-terminal constant region (amino…

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
Δ741-752-GFP

Plasmid

#33119

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γa3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of aa 741-752

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
Myo 9a-GFP R2166M

Plasmid

#134962

Purpose

Expression of rat Myo 9a (Myr 7) in mammalian cells.GAP minus mutant.

Depositor

Martin Bähler

Article

Chieregatti et al J Cell Sci. 1998 Dec 18;111 ( Pt 24):359

Insert

Myo 9a (rat) with 5' UTR. GAP minus mutant. (Myo9a Rat)

Tags

EGFP

Expression

Mammalian

Mutation

changed arginine 2166 to methionine

Promoter

CMV IE

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pDTnLAPP2A frame 1

Plasmid

#28227

Depositor

Harald von Melchner

Article

Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

Insert

Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein

Use

Retroviral

Tags

EGFP and nLAP-tag

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pARC (pKD-G3)

Plasmid

#62682

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP123

Use

RNAi

Expression

Mammalian

Available Since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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γA3/α1-GFP

Plasmid

#33112

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γa3 (Pcdhga3 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Cytoplaspic domain has been replaced with the cyt…

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pME-mir126new

Plasmid

#67712

Purpose

Replacement for 26033 776 pME-mir-126

Depositor

Nathan Lawson

Article

Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Insert

mir126a (mir126a Zebrafish)

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pCL0981

Plasmid

#252427

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 47-102 only

Promoter

CMV

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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scAAV-DDIO-GFP

Plasmid

#251348

Purpose

Cre and Flp dependent DTA (diptheria toxin) expressing system

Depositor

Samuel Pfaff

Article

Moore et al bioRxiv [Preprint]. 2025 Oct 18:2025.10.

Insert

Cre and Flp dependent eGFP driven by a EF1α(core) promoter

Use

AAV and Cre/Lox

Expression

Bacterial and Mammalian

Promoter

EF1α

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0918

Plasmid

#252411

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 1-46 only

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0946

Plasmid

#252416

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (H4C6 Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 1-20 only

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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sHiBiT-FLT1 Fusion Vector

Plasmid

#238589

Purpose

Express sHiBiT-FLT1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

FLT1 (FLT1 Human)

Tags

HiBiT

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGY240

Plasmid

#239811

Purpose

Express H3-EGFP and H2B-mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

H3
H2B

Use

Fungal expression

Tags

EGFP and mRFP1

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY251

Plasmid

#239813

Purpose

Express H2B-NLS-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

NLS of H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY219

Plasmid

#239806

Purpose

Express H2B-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H2B

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY220

Plasmid

#239807

Purpose

Express H3-EGFP in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Insert

H3

Use

Fungal expression

Tags

EGFP

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGY232

Plasmid

#239810

Purpose

Express EGFP-P2A-mRFP1 in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Tags

2A

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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