We narrowed to 16,183 results for: nes
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Plasmid#168465PurposeFor the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNLS-mTurquoise2-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-SpCas9-HF1
Plasmid#126770PurposeExpression of increased fidelity SpCas9-HF1 in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDYSCKO-ADE1
Plasmid#120264PurposepDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locusDepositorInsertpDYSCKO cassette-ADE1 targeting guide
UseCRISPR and Synthetic BiologyPromoterSNR52Available sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHCas9-Nours
Plasmid#107733PurposeEscherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiaeDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionYeastMutationHuman optimizedPromoterTEF1Available sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eBE-S1
Plasmid#101092PurposeExpresses eBE-S1 in mammalian cellsDepositorInserteBE-S1
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
U371-GFP ILtoEK
Plasmid#24472DepositorInsertUnc104 (unc-104 Nematode)
TagsGFPExpressionBacterialMutationContains only the kinesin's motor head fused…Available sinceApril 23, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS414-54
Plasmid#23084DepositorInsertyeast Histone H3-2 and Histone H4-2
ExpressionYeastMutationHistone H3 changed Lysines 4 and 9 to Glutamines,…Available sinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-I Y248&249F
Plasmid#22189DepositorInsertTFII-I (GTF2I Human)
TagsGSTExpressionMammalianMutationChanged Tyrosines 248 and 249 to Phenylalanines.Available sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-FastFUCCI-P2A-H2A-tdiRFP (JDW 1402)
Plasmid#242591PurposeA CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an H2A-tdiRFP for nuclear labeling.DepositorInsertmKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
ExpressionMammalianPromoterCAGGSAvailable sinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
UAS:syp-dL5-mNeonGreen
Plasmid#241798PurposeExpresses synaptically localized dL5 protein fused to mNeonGreen, under control of UAS promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseZebrafish transgenesis (tol1)Available sinceJuly 25, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT/CAGGS-mVenus-P2A-NRASG12V
Plasmid#236072PurposeSleeping Beauty transposon plasmid for hydrodynamic tail vein injection (HDTVi) into mice. Caggs promotor driven expression of mVenus and mutant NRAS (NRASG12V).DepositorInsertsUseSleeping beauty transposon plasmidMutationG12VPromoterCAGGSAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJC.15A.sgRNA.TA
Plasmid#232483PurposesgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.DepositorInsertsToxin-antitoxin System from CD630
medium-copy-number p15A origin of replication
mCherry
UseCRISPRExpressionBacterialPromoterP3 constitutive promoter and native promoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
WNV isolate WN NY 2000-crow3356 NS5 RdRp full length
Plasmid#228662PurposeBacterial expression of the West Nile virus NS5 RdRp protein for biochemical assayDepositorInsertWest nile NS5 RdRp
TagsHis-SUMOExpressionBacterialAvailable sinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)
Plasmid#229845PurposeA CAGGS-driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling followed by an IRES and a myristoylated TagBFP for labeling the cell membrane.DepositorInsertLamin nanobody
ExpressionMammalianAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP11980 - pAAV-DLX2.0-minRho-dTomato-10aa-H2B-WPRE3-BGHpA (Alias: CN1980)
Plasmid#229934PurposeDLX2.0 is an enhancer sequence, designed to drive nuclear dTomato expression in forebrain GABAergic neuronsDepositorInsertdTomato-10aa-H2B
UseAAVAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-Lifeact-EGFP-3xHA (JDW 1322)
Plasmid#224494PurposeGateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLiefact-EGFP-3xHA
UseGateway CloningAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)
Plasmid#208395PurposeTo generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.DepositorInsertHuman MRE11 (MRE11 Human)
UseLuciferase and RetroviralExpressionMammalianMutationDB1delta/DB2deltaAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA-Cre-GpNLuc-sgmSTING
Plasmid#208386PurposeLentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.DepositorAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TIE-V5/HIS
Plasmid#205612PurposeExpression of human TIE receptor tyrosine kinase in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTIE receptor tyrosine kinase (TIE1 Human)
TagsV5-HisExpressionMammalianPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKUNOC-ErbB1-mScarlet
Plasmid#197360PurposeEncoding human ErbB1 tagged with mScarlet.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERBB1 (EGFR Human)
TagsmScarletExpressionMammalianAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only