We narrowed to 19,676 results for: Cre

1. NTSR1-DuET

   Plasmid

   #213356

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   NTSR1 (NTSR1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS L30 Ruby3-Rab6A

   Plasmid

   #135653

   Purpose

   Rab6A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

   Depositor

   Raphael Gaudin

   Article

   Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

   Insert

   Rab6A
   

   Tags

   Ruby3

   Expression

   Mammalian

   Promoter

   L30

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCashGem-Xyl2-URA3-LINEAR

   Plasmid

   #174840

   Purpose

   pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Promoter

   ENO1p

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF-ManII-GFP

   Plasmid

   #160905

   Purpose

   Expresses the Golgi marker ManII-GFP in mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   MAN2A1 (MAN2A1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-SPA2G231V

   Plasmid

   #42079

   Depositor

   Christine Garcia

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2012 Dec 18;10

   Insert

   Surfactant Protein -A2 (SFTPA2 Human)
   

   Use

   Lentiviral; Plenti

   Tags

   V5

   Expression

   Bacterial and Mammalian

   Mutation

   Changed Glycine 231 to Valine

   Promoter

   CMV

   Available Since

   March 19, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAd MafA-I-nGFP

   Plasmid

   #19412

   Depositor

   Douglas Melton

   Article

   Zhou et al Nature. 2008 Oct 2;455(7213):627-32. doi

   Insert

   v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian) (Mafa Mouse)
   

   Use

   Adenoviral; Entry vector

   Tags

   GFP and IRES

   Available Since

   Nov. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pCRISPEY-GAL-Nat-ADE2

   Plasmid

   #232103

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. CA-0059 CMVTO-SumIL2-rens-5GS-IL2Rb

   Plasmid

   #231224

   Purpose

   CageIL-2 (C-term 25 AA)

   Depositor

   Xiaojing Gao

   Article

   Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

   Insert

   SumIL2-rens-5GS-IL2Rb (IL2 Human)
   

   Expression

   Mammalian

   Mutation

   L80F, R81D, L85V, I86V, I92F

   Available Since

   Jan. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330.sgNf1.4

   Plasmid

   #92028

   Purpose

   pX330 sgNf1.4 for Nf1 deletion

   Depositor

   Wen Xue

   Article

   Song et al Gastroenterology. 2017 Apr;152(5):1161-1

   Insert

   sgNf1.4
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

10. Sema6c-AP-His

    Plasmid

    #72041

    Purpose

    Expresses the extracellular region of the Sema6C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6c (Sema6c Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. AWP-031

    Plasmid

    #213967

    Purpose

    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

    Depositor

    Alexander Westermann

    Article

    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

    Insert

    Cas12a (cas12a Synthetic, Segatella bryantii)
    

    Tags

    3XFLAG

    Expression

    Bacterial

    Mutation

    D875A to inactivate target nuclease activity

    Promoter

    PcfxA, IPTG inducible

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV7.1_3xFLAG-STK4

    Plasmid

    #172989

    Purpose

    constitutive expression of FLAG-tagged STK4

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-STK4 (STK4 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcg 2.0-BFP-circ-arRNA backbone

    Plasmid

    #213965

    Purpose

    pcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences o

    Depositor

    Wensheng Wei

    Article

    Yi et al Nat Biotechnol. 2022 Jun;40(6):946-955.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sema6d.4-AP-His

    Plasmid

    #72044

    Purpose

    Expresses the extracellular region of the Sema6D, isoform 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema6d.4 (Sema6d Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. LacI-pLacI-pII pm

    Plasmid

    #218099

    Purpose

    Phagemid that expresses LacI, and pII under the Lac promoter

    Depositor

    Kevin Esvelt

    Insert

    pII
    

    Expression

    Bacterial

    Promoter

    Lac promoter

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYSD313

    Plasmid

    #212931

    Purpose

    Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    Sed1 (SED1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

    Plasmid

    #162335

    Purpose

    Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT9752-BEAR-mScarlet

    Plasmid

    #162991

    Purpose

    BEAR target plasmid with split mScarlet and disrupted 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    mScarlet split with an intron between amino acids 110-111
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    disrupted 5' splice site

    Promoter

    CMV

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-Blast-sgNT

    Plasmid

    #138678

    Purpose

    Expresses a Non-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgNT
    

    Use

    Lentiviral

    Promoter

    hU6

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. EF1a_SRY_P2A_Hygro_Barcode

    Plasmid

    #120485

    Purpose

    Barcoded lentiviral vector to express SRY in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    SRY (SRY Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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