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Unconventional secretion of alpha-synuclein mediated by palmitoylated DNAJC5 oligomers.
Wu S, Hernandez Villegas NC, Sirkis DW, Thomas-Wright I, Wade-Martins R, Schekman R
Elife. 2023 Jan 10;12:e85837. doi: 10.7554/eLife.85837.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
205705BFP-Rab5CA (Q79L)Mammalian expression of BFP-tagged mutant Rab5CA
205706pCMV-αS-2Mammalian expression of alpha-synuclein tandem dimer
205708pCMV-DNAJC5-L115RMammalian expression of DNAJC5 L115R
205709pCMV-DNAJC5-L116ΔMammalian expression of DNAJC5 L116Δ
205710pCMV-DNAJC5 (WT)-HaloTagMammalian expression of DNAJC5 (WT)-HaloTag
205711pCMV-DNAJC5 (L115R)-HaloTagMammalian expression of DNAJC5 (L115R)-HaloTag
205712pCMV-XPACK-DNAJC5 (L115R)- HaloTagMammalian expression of XPACK-DNAJC5 (L115R)-HaloTag
205713mNeonGreen-α-synucleinMammalian expression of mNeonGreen-alpha-synuclein
2057143H-α-synucleinMammalian expression of 3H-alpha-synuclein
205715α-synuclein-3HMammalian expression of alpha-synuclein-3H
205716pCMV-XPACK-DNAJC5 (WT)Mammalian expression of XPACK-DNAJC5 (WT)
205717pCMV-XPACK-DNAJC5 (L115R)Mammalian expression of XPACK-DNAJC5 (L115R)
205718pCMV-DEAD XPACK-DNAJC5 (L115R)Mammalian expression of DEAD XPACK-DNAJC5 (L115R)
205719pCMV-DNAJC5-ΔJ (Δ14-82)Mammalian expression of DNAJC5-ΔJ (Δ14-82)
205720pCMV-DNAJC5-ΔC10 (Δ189-198)Mammalian expression of DNAJC5-ΔC10 (Δ189-198)
205721pCMV-DNAJC5-ΔC20 (Δ179-198)Mammalian expression of DNAJC5-ΔC20 (Δ179-198)
205722pCMV-DNAJC5-ΔC30 (Δ169-198)Mammalian expression of DNAJC5-ΔC30 (Δ169-198)
205723pCMV-DNAJC5-ΔC40 (Δ151-198)Mammalian expression of DNAJC5-ΔC40 (Δ151-198)
205724pCMV-pOTC-GFPExpression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase
205725pCMV-pOTC-3H-GFPExpression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle
205727pLenti-CMV-DNAJC5Mammalian expression of DNAJC5
205728pLenti-CMV-DNAJC5 (L115R)Mammalian expression of DNAJC5 (L115R)
205729plKO.1-DNAJC5-ShRNAKnockdown of DNAJC5

Antibodies from Article