Unconventional secretion of alpha-synuclein mediated by palmitoylated DNAJC5 oligomers.
Wu S, Hernandez Villegas NC, Sirkis DW, Thomas-Wright I, Wade-Martins R, Schekman R
Elife. 2023 Jan 10;12:e85837. doi: 10.7554/eLife.85837. PubMed Article
Wu S, Hernandez Villegas NC, Sirkis DW, Thomas-Wright I, Wade-Martins R, Schekman R
Elife. 2023 Jan 10;12:e85837. doi: 10.7554/eLife.85837. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 205705 | BFP-Rab5CA (Q79L) | Mammalian expression of BFP-tagged mutant Rab5CA |
| 205706 | pCMV-αS-2 | Mammalian expression of alpha-synuclein tandem dimer |
| 205708 | pCMV-DNAJC5-L115R | Mammalian expression of DNAJC5 L115R |
| 205709 | pCMV-DNAJC5-L116Δ | Mammalian expression of DNAJC5 L116Δ |
| 205710 | pCMV-DNAJC5 (WT)-HaloTag | Mammalian expression of DNAJC5 (WT)-HaloTag |
| 205711 | pCMV-DNAJC5 (L115R)-HaloTag | Mammalian expression of DNAJC5 (L115R)-HaloTag |
| 205712 | pCMV-XPACK-DNAJC5 (L115R)- HaloTag | Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag |
| 205713 | mNeonGreen-α-synuclein | Mammalian expression of mNeonGreen-alpha-synuclein |
| 205714 | 3H-α-synuclein | Mammalian expression of 3H-alpha-synuclein |
| 205715 | α-synuclein-3H | Mammalian expression of alpha-synuclein-3H |
| 205716 | pCMV-XPACK-DNAJC5 (WT) | Mammalian expression of XPACK-DNAJC5 (WT) |
| 205717 | pCMV-XPACK-DNAJC5 (L115R) | Mammalian expression of XPACK-DNAJC5 (L115R) |
| 205718 | pCMV-DEAD XPACK-DNAJC5 (L115R) | Mammalian expression of DEAD XPACK-DNAJC5 (L115R) |
| 205719 | pCMV-DNAJC5-ΔJ (Δ14-82) | Mammalian expression of DNAJC5-ΔJ (Δ14-82) |
| 205720 | pCMV-DNAJC5-ΔC10 (Δ189-198) | Mammalian expression of DNAJC5-ΔC10 (Δ189-198) |
| 205721 | pCMV-DNAJC5-ΔC20 (Δ179-198) | Mammalian expression of DNAJC5-ΔC20 (Δ179-198) |
| 205722 | pCMV-DNAJC5-ΔC30 (Δ169-198) | Mammalian expression of DNAJC5-ΔC30 (Δ169-198) |
| 205723 | pCMV-DNAJC5-ΔC40 (Δ151-198) | Mammalian expression of DNAJC5-ΔC40 (Δ151-198) |
| 205724 | pCMV-pOTC-GFP | Expression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase |
| 205725 | pCMV-pOTC-3H-GFP | Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle |
| 205727 | pLenti-CMV-DNAJC5 | Mammalian expression of DNAJC5 |
| 205728 | pLenti-CMV-DNAJC5 (L115R) | Mammalian expression of DNAJC5 (L115R) |
| 205729 | plKO.1-DNAJC5-ShRNA | Knockdown of DNAJC5 |