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pCMV-DNAJC5 (L115R)-HaloTag
(Plasmid #205711)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 205711 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DNAJC5
  • Species
    H. sapiens (human)
  • Mutation
    L115R
  • Entrez Gene
    DNAJC5 (a.k.a. CLN4, CLN4B, CSP, DNAJC5A, NCL, mir-941-2, mir-941-3, mir-941-4, mir-941-5)
  • Tag / Fusion Protein
    • HaloTag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-DNAJC5 (L115R)-HaloTag was a gift from Randy Schekman (Addgene plasmid # 205711 ; http://n2t.net/addgene:205711 ; RRID:Addgene_205711)
  • For your References section:

    Unconventional secretion of alpha-synuclein mediated by palmitoylated DNAJC5 oligomers. Wu S, Hernandez Villegas NC, Sirkis DW, Thomas-Wright I, Wade-Martins R, Schekman R. Elife. 2023 Jan 10;12:e85837. doi: 10.7554/eLife.85837. 10.7554/eLife.85837 PubMed 36626307