3H-α-synuclein
(Plasmid #205714)

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Purpose

Mammalian expression of 3H-alpha-synuclein
Depositing Lab

Randy Schekman
Publication

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.7554/eLife.85837. ( How to cite )
Sequence Information
- Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.

Full plasmid sequence is not available for this item.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	205714	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Pending

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pCMV
Vector type
Mammalian Expression

Growth in Bacteria

Bacterial Resistance(s)
Kanamycin, 50 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
Unknown

Gene/Insert

Gene/Insert name
alpha-synuclein
Species
H. sapiens (human)
Entrez Gene
SNCA (a.k.a. NACP, PARK1, PARK4, PD1)
Tag / Fusion Protein
- thermostable three helix-bundle protein 3H (N terminal on insert)

Cloning Information

Cloning method Gibson Cloning

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

3H-α-synuclein was a gift from Randy Schekman (Addgene plasmid # 205714)
For your References section:

Unconventional secretion of alpha-synuclein mediated by palmitoylated DNAJC5 oligomers. Wu S, Hernandez Villegas NC, Sirkis DW, Thomas-Wright I, Wade-Martins R, Schekman R. Elife. 2023 Jan 10;12:e85837. doi: 10.7554/eLife.85837. 10.7554/eLife.85837 PubMed 36626307

3H-α-synuclein (Plasmid #205714)