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pENTR L5-GAL4-L2

Plasmid

#32304

Depositor

Steve Stowers

Article

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

GAL4 (GAL4 Budding Yeast)

Use

Gateway multisite entry clone

Available Since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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pGMCZ-PrpoB

Plasmid

#27073

Depositor

Dirk Schnappinger

Article

Kim et al Nucleic Acids Res. 2011 Mar;39(6):2210-2

Insert

P-rpoB

Expression

Bacterial

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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SpRY-PCE(cm24Cre)

Plasmid

#242426

Purpose

for chromosome editing

Depositor

Caixia Gao

Article

Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

Insert

SpRY-PE, cm24Cre

Use

CRISPR

Expression

Plant

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbV-EGFP

Plasmid

#216161

Purpose

Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Type

Empty backbone

Use

Lentiviral

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pST39-Ubc13-MMS2

Plasmid

#184807

Purpose

Bacterial expression of yeast UEV-E2 complex

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Ubc13, MMS2

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-GK in PLJM60

Plasmid

#154207

Purpose

Lentiviral vector for stable expression of GK

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

GK (GK Human)

Use

Lentiviral

Tags

N terminal FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSMPUW_6.10_M3_Ef1a-HA-gateway-SV40

Plasmid

#128446

Purpose

lentiviral destination vector with Ef1a promoter, N-terminal HA tag, and SV40 promoter

Depositor

Lindy Barrett

Insert

Ef1a promoter, N-terminal HA tag, and SV40 promoter

Use

Lentiviral

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pSABEn

Plasmid

#133969

Purpose

Expresses ABEn CRISPR base editor and gRNA scaffold in Streptomyces

Depositor

Yuhui Sun

Article

Zhong et al bioRxiv 630137

Insert

ABEn

Use

CRISPR and Synthetic Biology

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0612

Plasmid

#133505

Purpose

Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0782

Plasmid

#133514

Purpose

Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX

Expression

Yeast

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJFRC-20XUAS-IVS-PhiC31

Plasmid

#133565

Purpose

Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Expression

Insect

Promoter

20X UAS

Available Since

Oct. 28, 2019

Availability

Academic Institutions and Nonprofits only

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p10xEGFP-N1

Plasmid

#122170

Purpose

Expression of 10xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

10xEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pREVTRE-AID

Plasmid

#121067

Purpose

Retrovirus vector expresses AID-fusion protein under TRE promoter and with hygromycin resistance selectio

Depositor

Randy Poon

Article

Ng et al Cell Cycle. 2018 Dec 23. doi: 10.1080/15

Type

Empty backbone

Use

Retroviral

Tags

AID

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDM1513

Plasmid

#108998

Purpose

safe haven knock-in plasmid for the act5 locus, N-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, n…

Tags

GFP

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1515

Plasmid

#109000

Purpose

safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, c…

Tags

GFP

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDM1514

Plasmid

#108999

Purpose

safe haven knock-in plasmid for the act5 locus, N-terminal mCherry tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Safe haven knock-in plasmid for the act5 locus, n…

Tags

mCherry

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mApple

Plasmid

#96934

Purpose

Retroviral plasmid containing mApple for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Apple

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB212

Plasmid

#68603

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pIC195

Plasmid

#69730

Purpose

Retroviral expression of Polo-Like Kinase 1 (Plk1)

Depositor

Iain Cheeseman

Insert

Polo-Like Kinase 1 (Plk1) (PLK1 Human)

Use

Retroviral

Tags

eYFP-TEV-S

Expression

Mammalian

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSB4A5 JQ929582

Plasmid

#38169

Depositor

Drew Endy

Article

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

mKate2
Superfolder GFP

Use

Synthetic Biology; Biobrick cloning vector

Available Since

July 28, 2014

Availability

Academic Institutions and Nonprofits only