Cell-free biosensors for rapid detection of water contaminants.
Jung JK, Alam KK, Verosloff MS, Capdevila DA, Desmau M, Clauer PR, Lee JW, Nguyen PQ, Pasten PA, Matiasek SJ, Gaillard JF, Giedroc DP, Collins JJ, Lucks JB
Nat Biotechnol. 2020 Jul 6. pii: 10.1038/s41587-020-0571-7. doi: 10.1038/s41587-020-0571-7. PubMed Article
Jung JK, Alam KK, Verosloff MS, Capdevila DA, Desmau M, Clauer PR, Lee JW, Nguyen PQ, Pasten PA, Matiasek SJ, Gaillard JF, Giedroc DP, Collins JJ, Lucks JB
Nat Biotechnol. 2020 Jul 6. pii: 10.1038/s41587-020-0571-7. doi: 10.1038/s41587-020-0571-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140371 | pJBL701 | Expresses TetR protein under the control of T7-lacO promoter |
| 140372 | pJBL702 | Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 0BP dowsntream from the promoter |
| 140373 | pJBL703 | Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter |
| 140374 | pJBL704 | Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter |
| 140375 | pJBL705 | Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter |
| 140376 | pJBL706 | Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 6BP dowsntream from the promoter |
| 140377 | pJBL707 | Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 8BP dowsntream from the promoter |
| 140378 | pJBL708 | Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 10BP dowsntream from the promoter |
| 140379 | pJBL709 | Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 12BP dowsntream from the promoter |
| 140380 | pJBL710 | Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 14BP dowsntream from the promoter |
| 140381 | pJBL711 | Expresses OtrR protein under the control of T7-lacO promoter |
| 140382 | pJBL712 | Expresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoter |
| 140383 | pJBL713 | Expresses CtcS protein under the control of T7-lacO promoter |
| 140384 | pJBL714 | Expresses CtcS-regulated 3WJdB RNA aptamer driven by T7 promoter with the ctcO sequence 5BP downstream from the promoter |
| 140385 | pJBL715 | Expresses MphR protein under the control of T7-lacO promoter |
| 140386 | pJBL716 | Expresses MphR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mphO sequence 5BP downstream from the promoter |
| 140387 | pJBL717 | Expresses MobR protein under the control of T7-lacO promoter |
| 140388 | pJBL718 | Expresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoter |
| 140389 | pJBL719 | Expresses QacR protein under the control of T7-lacO promoter |
| 140390 | pJBL720 | Expresses QacR-regulated 3WJdB RNA aptamer driven by T7 promoter with the qacO sequence 5BP downstream from the promoter |
| 140391 | pJBL721 | Expresses TtgR protein under the control of T7-lacO promoter |
| 140392 | pJBL722 | Expresses TtgR-regulated 3WJdB RNA aptamer driven by T7 promoter with the ttgO sequence 5BP downstream from the promoter |
| 140393 | pJBL723 | Expresses HucR protein under the control of T7-lacO promoter |
| 140394 | pJBL724 | Expresses HucR-regulated 3WJdB RNA aptamer driven by T7 promoter with the hucO sequence 5BP downstream from the promoter |
| 140395 | pJBL725 | Expresses SmtB protein under the control of T7-lacO promoter |
| 140396 | pJBL726 | Expresses SmtB-regulated 3WJdB RNA aptamer driven by T7 promoter with the smtO sequence 5BP downstream from the promoter |
| 140397 | pJBL727 | Expresses CsoR protein under the control of T7-lacO promoter |
| 140398 | pJBL728 | Expresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoter |
| 140399 | pJBL729 | Expresses CadC-regulated 3WJdB RNA aptamer driven by T7 promoter with the cadO sequence 5BP downstream from the promoter |