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pT7-EGFP-C1-HsNot1iso2-del1844-2133_T

Plasmid

#147628

Purpose

Mammalian Expression of HsNot1iso2-del1844-2133

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot1iso2-del1844-2133 (CNOT1 Human)

Expression

Mammalian

Mutation

4 silent and two non silent G917E and R2353Q muta…

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NvG-HsNot2_344-540_T

Plasmid

#147618

Purpose

Bacterial Expression of HsNot2_344-540

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_344-540 (CNOT2 Human)

Expression

Bacterial

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-F502A_S

Plasmid

#147501

Purpose

Mammalian Expression of HsNot2-F502A

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2-F502A (CNOT2 Human)

Expression

Mammalian

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pEMF034

Plasmid

#157631

Purpose

sMHT-113

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17
SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDH009

Plasmid

#157644

Purpose

sMHT-113 fused to GRX2

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-GRX2
GRX2-sMHT(114-230)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF035

Plasmid

#157632

Purpose

sMHT-67

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-67)-SYNZIP17
SYNZIP18-sMHT(68-230)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF036

Plasmid

#157633

Purpose

sMHT-129

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-129)-SYNZIP17
SYNZIP18-sMHT(130-230)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF038

Plasmid

#157635

Purpose

Empty vector

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Type

Empty backbone

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF047

Plasmid

#157636

Purpose

sMHT-113 F1 only

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

sMHT(1-113)-SYNZIP17

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF044

Plasmid

#157637

Purpose

sMHT-113 F2 only

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDH001

Plasmid

#157639

Purpose

Batis maritima MHT

Depositor

Jonathan Silberg

Publication

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Insert

MHT

Expression

Bacterial

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRB30-GFP

Plasmid

#165481

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with C-terminal uv-GFP, Flag and His

Depositor

Renato Bruni

Publication

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

TEV-GFP-FLAG-10x His

Expression

Mammalian

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

Plasmid

#157674

Purpose

Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

Depositor

Michael Nash

Publication

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

SpyCatcher with ddFLN4, ELP, Histag and ybbR tag

Tags

ddFLN4, ELP, Histag and ybbR tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod-Doc(WT)

Plasmid

#157675

Purpose

Expression of XMod-Doc wild type with His6x tag and SpyTag.

Depositor

Michael Nash

Publication

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc Wild type with Histag and SpyTag

Tags

Histag and SpyTag

Expression

Bacterial

Promoter

T5

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-Coh-His-SpyTag

Plasmid

#157673

Purpose

Expression of Coh (type III from Ruminococcus flavefaciens) with His6x tag and SpyTag.

Depositor

Michael Nash

Publication

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

Cohesin (R.f) with Histag and SpyTag

Tags

Histag and SpyTag

Expression

Bacterial

Promoter

T5

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pXWSPecf11

Plasmid

#160841

Purpose

1 repeat of Pecf11 sponge

Depositor

Baojun Wang

Publication

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

Pecf11-t

Use

Synthetic Biology

Promoter

Pecf11

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pXWS40LBS

Plasmid

#160839

Purpose

40 repeats of LBS sponge

Depositor

Baojun Wang

Publication

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(40 X LBS)-t

Use

Synthetic Biology

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pXWS80LBS

Plasmid

#160840

Purpose

80 repeats of LBS sponge

Depositor

Baojun Wang

Publication

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(80 X LBS)-t

Use

Synthetic Biology

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pXWS10Pecf11

Plasmid

#160842

Purpose

10 repeats of Pecf11 sponge

Depositor

Baojun Wang

Publication

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(10 X Pecf11)-t

Use

Synthetic Biology

Promoter

Pecf11

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pXWS20Pecf11

Plasmid

#160843

Purpose

20 repeats of Pecf11 sponge

Depositor

Baojun Wang

Publication

Wan et al Nat Commun. 2020 Nov 24;11(1):5961. doi:

Insert

(20 X Pecf11)-t

Use

Synthetic Biology

Promoter

Pecf11

Available since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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