We narrowed to 16,131 results for: form

1. pLP-PIGA2 (pLP300)

   Plasmid

   #168462

   Purpose

   Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.

   Depositor

   Matthew Maurano

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Type

   Empty backbone

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pαH-S-HRV3C

   Plasmid

   #164567

   Purpose

   SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike - Furin site changed for HRV3C site
   

   Tags

   2X Strep-Tag II and 8X His tag

   Expression

   Mammalian

   Mutation

   682-685 (furin site) replaced with HRV3C site (LE…

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUW-TetO-BATF

   Plasmid

   #178451

   Purpose

   doxycycline-inducible expression of Human BATF in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   BATF (BATF Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTWIN1-His6-Ssp-Httex1-7Q

   Plasmid

   #84347

   Purpose

   Expression of the human Huntingtin Exon1 protein containing 7Q in E.coli

   Depositor

   Hilal Lashuel

   Article

   Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

   Insert

   Huntingtin Exon 1 (HTT Human)
   

   Tags

   N-terminal His6-tagged intein Ssp

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPGK-T7/2-CD44v6 (-cyt)

   Plasmid

   #137817

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6 (-cyt) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region, R296K (please see dep…

   Promoter

   PGK

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. TetO-FUW-Mecom 1

   Plasmid

   #118590

   Purpose

   Expresses Mecom isoform 1 from the TRE3G promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Mecom (Mecom Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-mDrc7-FLAG

   Plasmid

   #176470

   Purpose

   Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

   Depositor

   Masahito Ikawa

   Article

   Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

   Insert

   dynein regulatory complex subunit 7 (Drc7 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP4-BG505.SOSIP-QES.i03.c01-TM

   Plasmid

   #111845

   Purpose

   Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether; QES mutant with enhanced binding to PG16

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Tags

   CD5 leader sequence and TM helix from MHC class I

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

   Plasmid

   #178851

   Purpose

   Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)
   

   Expression

   Mammalian

   Mutation

   Gαq: C9S/C10S/Q209L/L254A

   Promoter

   CAG

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF0062

    Plasmid

    #141503

    Purpose

    Lentiviral vector for overexpressing the CTBP2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    CTBP2 (CTBP2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_Tau Authentic Alu 10-12V337M

    Plasmid

    #194169

    Purpose

    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 V337M
    

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    Changed Valine 337 to Methinonine

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ai95(RCL-GCaMP6f) targeting vector

    Plasmid

    #61579

    Purpose

    Target a Cre-dependent GCaMP6-fast expression cassette to the mouse Rosa26 locus

    Depositor

    Hongkui Zeng

    Article

    Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

    Insert

    CAG-LSL-GCaMP6-fast
    

    Use

    Cre/Lox and Mouse Targeting

    Promoter

    CAG

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-mNG-eDHFR(69K6)-G-alpha-q

    Plasmid

    #172106

    Purpose

    Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)
    

    Expression

    Mammalian

    Mutation

    Gαq: C9S/C10S/Q209L

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmx-puro-MiGT

    Plasmid

    #172399

    Purpose

    Retroviral expression vector for murine direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Wang et al Cells. 2020 Jan 22;9(2). pii: cells90202

    Insert

    Mef2c-i, Gata4, Tbx5 (Gata4, Tbx5, Mef2c Mouse)
    

    Use

    Retroviral

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SITS-mCherry-Bin1b

    Plasmid

    #176025

    Purpose

    Leishmania cell free expression of zebrafish mCherry-Bin1b. Parton lab clone GSU

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    Bin1b (bin1b Zebrafish)
    

    Use

    Leishmania cell free expression

    Tags

    mCherry

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 (-) hYAP 1-1delta

    Plasmid

    #124142

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. VAPA/B scFv [N479/107]

    Plasmid

    #206742

    Purpose

    Mammalian Expression of VAPA/B scFV. Derived from hybridoma N479/107 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    VAPA/B (Rattus norvegicus) recombinant scFV (Vapa Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)

    Plasmid

    #117856

    Purpose

    This plasmid expresses Kir2.1 along with fluorescent marker RFP under the control of CMV promoter. The vector also supports the construction of adenovirus using ADeasy system.

    Depositor

    C. Ron Yu

    Article

    Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Use

    Adenoviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSB63 - pL0_fLUC-I (CDS1)

    Plasmid

    #123180

    Purpose

    Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
    

    Use

    Luciferase; Part for plant expression

    Expression

    Plant

    Mutation

    No BpiI and BsaI sites

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Synaptotagmin-7 scFv [N275/14]

    Plasmid

    #211608

    Purpose

    Mammalian Expression Plasmid of Synaptotagmin-7 scFv. Derived from hybridoma N275/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Synaptotagmin-7 (Rattus norvegicus) recombinant mouse scFv. (Syt7 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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