We narrowed to 62,227 results for: cloning

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pGGD006

Plasmid

#48836

Purpose

Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 NLS

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGF004

Plasmid

#48845

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

p35S::D-AlanineR::t35S

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site in 35S promoter re…

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB005

Plasmid

#48822

Purpose

Provides the SV40 nuclear localization signal as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 nuclear localization signal

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB006

Plasmid

#48823

Purpose

Provides an ER signal sequence as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

signal sequence ER

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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C238-E02: attB2r-BGHpolyA-attB3

Plasmid

#162916

Purpose

Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Bovine growth hormone polyadenylation sequence

Use

Synthetic Biology

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E02: attB4-CAGp>-attB5

Plasmid

#162972

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the CAG promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

chicken beta-actin/CMV enhancer promoter

Use

Synthetic Biology

Available Since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E16: CyPet

Plasmid

#162896

Purpose

Standard Gateway entry clone encoding CyPet fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

CyPet fluorescent protein

Use

Synthetic Biology

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E17: mPlum

Plasmid

#162897

Purpose

Standard Gateway entry clone encoding mPlum fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Plum fluorescent protein

Use

Synthetic Biology

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRS143

Plasmid

#122377

Purpose

CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail)

Depositor

Rebecca Shapiro

Article

Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pTHSSe_52

Plasmid

#109250

Purpose

ColE1 BbsI cloning vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23105

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pRiboS EcoHind

Plasmid

#36254

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pRibo EcoHind

Plasmid

#36252

Depositor

Carolyn Bertozzi, Jessica Seeliger

Article

Seeliger et al PLoS One. 2012;7(1):e29266. Epub 2012 Ja

Type

Empty backbone

Use

Synthetic Biology; Mycobacteria replicating vector

Expression

Bacterial

Promoter

Modified Hsp60

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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pNIC-GST-TEV-GG

Plasmid

#215795

Purpose

Cloning of target gene with N terminal GST tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLV UBC APEX2-3xFLAG-IRES-eGFP

Plasmid

#214983

Purpose

Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

APEX2 (APX2 Glycine max (soybean))

Use

Lentiviral

Tags

3xFLAG

Promoter

UBC

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMLS280

Plasmid

#73738

Purpose

Simple cloning vector

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Type

Empty backbone

Use

Unspecified

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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Dendra1-C2

Plasmid

#54724

Purpose

Localization: C2 Cloning Vector, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Type

Empty backbone

Tags

Dendra1

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mGeos2-VEL-C1

Plasmid

#54541

Purpose

Localization: C1 Cloning Vector, Excitation: 503 / 569, Emission: 514 / 581

Depositor

Michael Davidson

Type

Empty backbone

Tags

mGeos2-VEL

Expression

Mammalian

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
wtKikGR-N1

Plasmid

#54718

Purpose

Localization: N1 Cloning Vector, Excitation: 507 / 583, Emission: 517 / 593

Depositor

Michael Davidson

Type

Empty backbone

Tags

wtKikGR

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
Phamret-N1

Plasmid

#54641

Purpose

Localization: N1 Cloning Vector, Excitation: 458 / 475, Emission: 458 / 517

Depositor

Michael Davidson

Type

Empty backbone

Tags

Phamret

Expression

Mammalian

Available Since

June 11, 2014

Availability

Industry, Academic Institutions, and Nonprofits
dEos-N1

Plasmid

#54497

Purpose

Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Type

Empty backbone

Tags

dEos

Expression

Mammalian

Available Since

June 11, 2014

Availability

Industry, Academic Institutions, and Nonprofits