We narrowed to 62,928 results for: cloning

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pGWB551

Plasmid

#74881

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Publication

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti-Trono-EFS-Blast-P2A-TurboRFP

Plasmid

#228893

Purpose

Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

Depositor

Francisco J Sánchez-Rivera

Publication

Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMC051-HisMS2_PLP_pac_LC

Plasmid

#128234

Purpose

Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

Depositor

Paul Freemont

Publication

de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

Inserts

Maturation Protein
Coat Protein Dimer

Expression

Bacterial

Promoter

T7

Available since

Nov. 15, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAM4951 (pCV0063)

Plasmid

#120084

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).

Depositor

Susan Golden

Publication

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG010

Plasmid

#113984

Purpose

Vector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectors

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

RK2 (IncP)

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TAL-L1-2.4-Ctrl

Plasmid

#103037

Purpose

Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by multi cloning site

Depositor

Maria-Elena Torres-Padilla

Publication

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-2.4

Use

T3 in vitro transcription for expression in mamma…

Tags

FLAG and HA

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAP699e

Plasmid

#253943

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Publication

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Linker::T2A::Linker::GCamp6s::Linker+CAAX

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAP826e

Plasmid

#253946

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Publication

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Begin-mNeonGreen::PH-domain::Linker::mCarmine::Linker::T2A::Linker

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAP311

Plasmid

#253947

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Publication

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Linker::3xFlag::mVenus::HAtag::Linker

Available since

May 20, 2026

Availability

Academic Institutions and Nonprofits only

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pAP696e

Plasmid

#246042

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Publication

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Linker::T2A::Linker::mNeonGreen::Linker+CAAX

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pAP702e

Plasmid

#253945

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Publication

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Begin-mNeonGreen:: SV40-NLS::Linker::Nucleoplasmin-NLS::mScarlet::Linker::T2A::Linker

Available since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pAP701e

Plasmid

#253942

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Publication

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Begin-mScarlet::SV40-NLS::Linker::Nucleoplasmin-NLS::mNeonGreen::Linker::T2A::Linker

Available since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pAP697e

Plasmid

#253944

Purpose

PCR donor synthesis for endogenous gene tagging

Depositor

Alexandre Paix

Publication

Knoblich et al Bio Protoc. 2025 Jun 20;15(12):e5360. do

Insert

Linker::T2A::Linker::mScarlet::Linker+CAAX

Available since

April 6, 2026

Availability

Academic Institutions and Nonprofits only

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pVP076

Plasmid

#222028

Purpose

Empty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.

Depositor

Wayne Parrott

Publication

Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do

Type

Empty backbone

Use

Synthetic Biology; Greengate compatible cloning v…

Mutation

All Esp3i sites domesticated

Available since

Dec. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRB33

Plasmid

#165482

Purpose

pTriEx based vector for protein expression in mammalian cells for targets fused with N-terminal Flag and His

Depositor

Renato Bruni

Publication

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Tags

10xHis-FLAG-TEV

Expression

Mammalian

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pFH131

Plasmid

#136550

Purpose

crRNA backbone for FnCas12a/CmsI nucleases, combine with OsU3 promoter module (pFH39)

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

crRNA backbone for FnCas12a/CmsI nucleases, combine with OsU3 promoter module (pFH39)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFH132

Plasmid

#136547

Purpose

Level1 ZmUBIp::FnCas12a (human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::FnCas12a (human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFH133

Plasmid

#136548

Purpose

Level1 ZmUBIp::LbCas12a (human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::LbCas12a (human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFH25

Plasmid

#131764

Purpose

Level0 SpCas9, wheat codon optimized (version 3)

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SpCas9, wheat codon optimized, version 3

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAK010

Plasmid

#128227

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 4

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 4

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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