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Efg1-ΔDBD-Y478S/Y482S/Y484S

Plasmid

#216411

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

Depositor

Nicolas Fawzi

Publication

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD-Y478S/Y482S/Y484S

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 478, 482 and 484 to Serine, and …

Available since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-myr-rsEGFP2- LDLR

Plasmid

#213399

Purpose

double floxed, myristoylation site (myr) and LDLR, fused to reversibly switchable EGFP rsEGFP2

Depositor

Katrin Willig

Publication

Willig et al Cell Rep. 2021 Jun 1;35(9):109192. doi:

Insert

rsEGFP2

Use

AAV

Tags

C-terminal (Ct) cytoplasmic domains of low densit…

Expression

Mammalian

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TST-EGFP-GPIDAF

Plasmid

#213708

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIDAF driven by an hPGK promoter.

Depositor

Qilai Huang

Publication

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

hPGK

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TST-EGFP-GPIBY55

Plasmid

#213707

Purpose

For lentiviral expression of shRNA under a U6 promoter, accompanied by the expression of the affinity sorting tag TST-EGFP-GPIBY55 driven by an hPGK promoter.

Depositor

Qilai Huang

Publication

Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:

Insert

EGFP

Tags

Twin-strep-tag

Expression

Mammalian

Promoter

hPGK

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pBad33_ISRor9_reRNA

Plasmid

#205352

Purpose

Bacterial expression of ISRor9 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISRor9 TnpB

Expression

Bacterial

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBad33_ISNsp3_reRNA

Plasmid

#205351

Purpose

Bacterial expression of ISNsp3 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISNsp3 TnpB

Expression

Bacterial

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBad33_ISCpe2_reRNA

Plasmid

#205335

Purpose

Bacterial expression of ISCpe2 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISCpe2 TnpB

Expression

Bacterial

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pBad33_ISCbt1_reRNA

Plasmid

#205330

Purpose

Bacterial expression of ISCbt1 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISCbt1 TnpB

Expression

Bacterial

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Il13 mutDRACH

Plasmid

#207130

Purpose

Luciferase vector containing 3'UTR for mouse Il13 with mutated DRACH sites

Depositor

Silvia Monticelli

Publication

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Interleukin 13 (Il13) 3'UTR (Il13 Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

TGAGGAGAGACCATCCCTGGGCATCTCAGCTGTGGACTCATTTTCCTTT…

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

Plasmid

#207135

Purpose

DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

Depositor

Silvia Monticelli

Publication

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)

Expression

Mammalian

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

Plasmid

#207136

Purpose

DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

Depositor

Silvia Monticelli

Publication

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)

Expression

Mammalian

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSCALPS-FLAG-HA-mmuMettl3-LN

Plasmid

#207129

Purpose

Lentiviral plasmid to express mouse Mettl3 Lambda peptide C-ter

Depositor

Silvia Monticelli

Publication

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Mettl3 (Mettl3 Mouse)

Use

Lentiviral

Tags

FLAG-HA and Lambda Peptide (LN)

Promoter

SSFV

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISAeme8_reRNA

Plasmid

#205324

Purpose

Bacterial expression of ISAeme8 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAeme8 TnpB

Expression

Bacterial

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISAepa1_reRNA

Plasmid

#205325

Purpose

Bacterial expression of ISAepa1 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAepa1 TnpB

Expression

Bacterial

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISBce3_reRNA

Plasmid

#205327

Purpose

Bacterial expression of ISBce3 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISBce3 TnpB

Expression

Bacterial

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISBth16_reRNA

Plasmid

#205328

Purpose

Bacterial expression of ISBth16 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISBth16 TnpB

Expression

Bacterial

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISCath1_reRNA

Plasmid

#205329

Purpose

Bacterial expression of ISCath1 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISCath1 TnpB

Expression

Bacterial

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISCbt2_reRNA

Plasmid

#205331

Purpose

Bacterial expression of ISCbt2 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISCbt2 TnpB

Expression

Bacterial

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISClte2_reRNA

Plasmid

#205334

Purpose

Bacterial expression of ISClte2 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISClte2 TnpB

Expression

Bacterial

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pBad33_ISEc44_reRNA

Plasmid

#205342

Purpose

Bacterial expression of ISEc44 TnpB and its reRNA

Depositor

Haoyi Wang

Publication

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISEc44 TnpB

Expression

Bacterial

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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