We narrowed to 12,188 results for: LEA
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Plasmid#240518PurposeMammalian expression of AcrIIC3-mCherry fusion with restriction enzyme sites in the 3' UTR for use as microRNA repression. BFP expressed separately as a transfection control.DepositorInsertAcrIIC3
TagsFLAG and mCherryExpressionMammalianPromoterCMVAvailable sinceAug. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTE5425
Plasmid#229032PurposeExpression vector for 10xHis-phi29 DNA polymerase (catalytically inactive mutant D249E): PT7-lacO-g10leader-p2 dead-T7 terminator, CamRDepositorInsertPT7-lacO-g10lead-10xHisTag-phi29 DNAP (D249E, catalytically inactive)
Tags10x His-tag + Factor Xa recognition siteExpressionBacterialPromoterPT7-lacO and T7 promoterAvailable sinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP
Plasmid#226114PurposeMammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control.DepositorInsertAcrIIC3
TagsFLAG and mCherryExpressionMammalianPromoterCMVAvailable sinceMay 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_FL Gcn4
Plasmid#232955PurposeGalactose iduced expression of full-length Gcn4 in yeastDepositorInsertGcn4
TagsTEV cleavage siteExpressionYeastPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 R2
Plasmid#232970PurposeGalactose iduced expression of Gcn4 R+ in yeastDepositorInsertGcn4 R+
TagsTEV cleavage siteExpressionYeastMutationK118R, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 L113A+
Plasmid#231873PurposeBacterial expression of N-terminally 6His tagged Gcn4 L113A+DepositorInsertGcn4 L113A+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationL113A, E114Y, N126H, D127E, S136APromoterT7Available sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 Aro3+
Plasmid#231884PurposeBacterial expression of N-terminally 6His tagged Gcn4 Aro3+DepositorInsertGcn4 Aro3+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationD103Y, N112T, L113Y, S117F, K140RPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDest17_Gcn4 Aro2+
Plasmid#231883PurposeBacterial expression of N-terminally 6His tagged Gcn4 Aro2+DepositorInsertGcn4 Aro2+
Tags6xHis, TEV cleavage siteExpressionBacterialMutationN116F, S117E, E119N, P129Q, T132YPromoterT7Available sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cdk13_intron3_sg4_pX458
Plasmid#127339PurposesgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #4DepositorAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTarget-Dual
Plasmid#207884PurposeCarries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.DepositorInsertsI-OnuI target site
I-GpeMI target site
UseSynthetic BiologyExpressionBacterialAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-PCi
Plasmid#214038PurposeBacterial expression plasmid for strep-tagged Pci from Haliangium ochraceumDepositorInsertPCi
TagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT-sm_PCaspase
Plasmid#214048PurposeBacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
ExpressionBacterialMutationR456E, D459K, D476K, R479EPromoterlacUV5 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-SAVED-CHAT
Plasmid#214030PurposeBacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceumDepositorInsertSAVED-CHAT
TagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJS-BCD-Strep-PC-σ
Plasmid#214037PurposeBacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceumDepositorInsertPC-σ
TagsStrep-tag IIExpressionBacterialMutationWTPromoterT7 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxEXPr6
Plasmid#218290PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3DepositorPublicationInserthph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
ExpressionYeastAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1314
Plasmid#217956PurposeExpression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
TagseGFPExpressionMammalianMutationGGG insertion between A95 and C96PromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_V47N_I48N
Plasmid#214319PurposeExpression of gene in mammalian cells by retroviral transductionDepositorAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_LES33AAA
Plasmid#214321PurposeExpression of gene in mammalian cells by retroviral transductionDepositorAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_DDEN29ENGD
Plasmid#214320PurposeExpression of gene in mammalian cells by retroviral transductionDepositorAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-human-pro-IL-18_E192K_D193K
Plasmid#214318PurposeExpression of gene in mammalian cells by retroviral transductionDepositorAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only