We narrowed to 16,131 results for: form

1. pdest METTL1-WT Neo

   Plasmid

   #223058

   Purpose

   METTL1 gene expression in mammalian cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   METTL1 (METTL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHRIG-Akt3

   Plasmid

   #53596

   Purpose

   lentiviral expression of constitutively active human Akt3 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   cAkt3 (AKT3 Human)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   constitutively active (myristoylated version)

   Promoter

   CMV

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

3. AAV phSyn1(S)-DreO-bGHpA

   Plasmid

   #50363

   Purpose

   Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Has Service

   AAV Retrograde and AAV5

   Insert

   DreO recombinase
   

   Use

   AAV

   Tags

   none

   Mutation

   Sequence optimized for expression in mammalian ce…

   Promoter

   hSyn1

   Available Since

   Jan. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1D V5-His-TOPO-KGA.wt

   Plasmid

   #110332

   Purpose

   Mammalian expression of glutaminase isoform KGA (wild type) with V5 and 6xHis tags

   Depositor

   Sandra Martha Gomes Dias

   Article

   Cassago et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

   Insert

   KGA glutaminase (GLS Human)
   

   Tags

   6xHis and V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. rag2:myr-Akt2_pISceI

   Plasmid

   #231495

   Purpose

   Expresses myristolated Akt2 in zebrafish lymphoid and mesenchymal cells

   Depositor

   Alejandro Gutierrez

   Article

   Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

   Insert

   Akt2 (Akt2 Mouse)
   

   Use

   Zebrafish expression

   Tags

   N-terminal myristolation signal

   Available Since

   June 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

   Plasmid

   #223159

   Purpose

   Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Slc17a7-IRES2-Cre targeting vector

   Plasmid

   #61574

   Purpose

   Target the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   Slc17a7-IRES2-Cre
   

   Use

   Cre/Lox and Mouse Targeting

   Available Since

   July 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-TrpC1 [1F1R]

   Plasmid

   #177437

   Purpose

   Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV pCAG-FLEX2-tTA2-WPRE-bGHpA

   Plasmid

   #65458

   Purpose

   Can be used to generate AAV virus that will express the tetracycline transactivator tTA2 from the CAG promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   tTA2
   

   Use

   AAV

   Promoter

   CAG

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Neuroligin-2 scFv [L107/95]

    Plasmid

    #206764

    Purpose

    Mammalian Expression of Neuroligin-2 scFV. Derived from hybridoma L107/95 scFv.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Neuroligin-2 (Mus musculus) recombinant scFV (Nlgn2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVD1 tRNA-gRNA position E2 (GB2239)

    Plasmid

    #160561

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tRNA-gRNA position E2 (Multiplexing Edit)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor

    Plasmid

    #229789

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mNgn2; ZF10-NS3-p65 (Neurog2 Mouse)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    ZF10 and CAG

    Available Since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Anti-GFP [N86/34R]

    Plasmid

    #220419

    Purpose

    Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p11d-tRPA (MSW#111)

    Plasmid

    #208066

    Purpose

    expression of human Replication Protein A in E. coli

    Depositor

    Marc Wold

    Article

    Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

    Insert

    RPA1, RPA2, RPA3 (RPA1, RPA3, RPA2 Human)
    

    Expression

    Bacterial

    Mutation

    RPA32 has a point mutation Thr88(ACC) -> Ile(A…

    Available Since

    Nov. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-SIN-BX SpyCatcher003-(short)mCD80

    Plasmid

    #210567

    Purpose

    Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid

    #115694

    Purpose

    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Inserts

    NmeCas9
    Nme-sgRNA
    

    Use

    CRISPR

    Tags

    NLS and NLS, HA

    Expression

    Insect and Mammalian

    Mutation

    None and fusion of repeat/tracrRNA to make a sgRNA

    Promoter

    EF-1 alpha and U6

    Available Since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. G protein alpha-s-YFP

    Plasmid

    #55781

    Purpose

    This G protein alpha-s construct contains internal insertions of YFP and the EE epitope.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    alpha-s-EE YFP (Gnas Rat)
    

    Tags

    EYFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    Met was substituted for Gln69 in EYFP (Clontech).…

    Promoter

    CMV

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6

    Plasmid

    #174498

    Purpose

    bacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN2 (SEPTIN2 Human)
    SEPTIN6 (SEPTIN6 Human)
    

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2-CIB1-mCherry-Rab8A

    Plasmid

    #194333

    Purpose

    For use in light-induced Rab8a protein inactivation through the interaction with CRY2

    Depositor

    Heidi Hehnly

    Article

    Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

    Inserts

    CIB1 (CIB1 Mustard Weed)
    Rab8a (RAB8A Canis Lupus)
    

    Tags

    mcherry

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti-Lb-Cas12a-2xNLS

    Plasmid

    #155046

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    LbCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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