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pLENTI_RPS3_K227/230R_Keima

Plasmid

#127146

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Mutation

K227R/K230R

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-zic2a Q

Plasmid

#89386

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

June 15, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9

Plasmid

#140237

Purpose

Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

EF-1a core

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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HA-Beclin 1

Plasmid

#86748

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Beclin 1 (BECN1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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ATG9A-EGFP

Plasmid

#210367

Purpose

Expresses ATG9A fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

ATG9A (ATG9A Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-Dvl2 gRNA

Plasmid

#246556

Purpose

CRISPR vector co-expressing Cas9 and a mouse Dvl2 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Dvl2 gRNA (Dvl2 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-Spike

Plasmid

#168911

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-Spike (S )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP2

Plasmid

#79874

Purpose

Bacillus subtilis sgRNA expression vector; integrates into amyE

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

sgRNA RR1

Use

CRISPR

Expression

Bacterial

Promoter

veg

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-Nucleocapsid

Plasmid

#168967

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-Nucleocapsid (N )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry GFP

Plasmid

#78535

Purpose

Control plasmid (paired gRNAs against GFP)

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HIS HSPA1A

Plasmid

#19537

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

heat shock 70kDa protein 1A (HSPA1A Human)

Tags

His

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-Cyclin-B

Plasmid

#177012

Purpose

Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

Cyclin B1 (CCNB1 Human)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OT1.0

Plasmid

#185387

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-MED17

Plasmid

#15410

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Insert

MED17 (MED17 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-BCR-ABL-p210-FLAG

Plasmid

#205619

Purpose

Expression of BCR-ABL oncogene in mammalian cells

Depositor

Pavel Krejčí

Article

Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

Insert

BCR-ABL oncogene, p210 isoform b3a2

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK

Plasmid

#25033

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

Rck (DDX6 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pLX-B2 (GB3374)

Plasmid

#160636

Purpose

pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

Depositor

Juan Antonio García, Fabio Pasin

Article

Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pC001 - huLshC2C2-MBP for bacterial expression

Plasmid

#79150

Purpose

Expresses human codon-optimized LshC2c2 for purification in E. coli

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

C2c2

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pBGPa-CMV-GFP-OSBP PH domain

Plasmid

#58724

Purpose

Expresses GFP-Tagged OSBP PH domain in mammalian cells

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Tags

GFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA5-H2B-AID-EYFP

Plasmid

#47329

Purpose

for plasmid integration using the Flp-In system. AID at C-terminal

Depositor

Don Cleveland

Article

Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

Insert

histone H2B (H2BC21 Human)

Tags

AID and EYFP

Expression

Mammalian

Available Since

Aug. 23, 2013

Availability

Academic Institutions and Nonprofits only

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