We narrowed to 9,922 results for: pET

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pAG415Gal_Gcn4 WW+GFP

Plasmid

#233003

Purpose

Galactose iduced expression of Gcn4 WW+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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ppAG415Gal_Gcn4 L113A+

Plasmid

#232972

Purpose

Galactose iduced expression of Gcn4 L113A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L113A

Tags

TEV cleavage site

Expression

Yeast

Mutation

L113A, E114Y, N126H, D127E, S136A

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 StoL

Plasmid

#232981

Purpose

Galactose iduced expression of Gcn4 StoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 StoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S104L, S117L, S136L, S144L

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAG415Gal_Gcn4 R3W

Plasmid

#232975

Purpose

Galactose iduced expression of Gcn4 R++ W+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R++ W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103R, N126W, D133R, K143R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAIP4_6WEY

Plasmid

#234234

Purpose

Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_5HIH

Plasmid

#234205

Purpose

Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_3ONH

Plasmid

#234179

Purpose

Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

3ONH (UBA2 Budding Yeast)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_5GUQ

Plasmid

#234171

Purpose

Heterologous protein expression of ASCH domain-containing ribonuclease in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

5GUQ

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_4QMD

Plasmid

#234174

Purpose

Heterologous protein expression of envoplakin in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

4QMD (EVPL Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_2H3L

Plasmid

#234160

Purpose

Heterologous protein expression of erbin (Densin-180-like protein) (Erbb2-interacting protein) in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

2H3L (ERBIN Human)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_5EF9

Plasmid

#234154

Purpose

Heterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

5EF9 (PB2 Influenza B virus (B/Lee/1940))

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_2H2Z

Plasmid

#234147

Purpose

Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli

Depositor

Christopher Bahl

Publication

Cárcamo Noriega et al Protein Sci. 2026 Sep;35(9):e70746. doi:

Insert

2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pINQ-ON10

Plasmid

#198689

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

Depositor

Gualberto González Sapienza

Publication

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody ON10

Tags

6xHis tag, HA tag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJB53

Plasmid

#226309

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1-3

Expression

Bacterial

Mutation

VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) T2C, C90A, T198C

Plasmid

#220235

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Publication

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

T2C, C90A, T198C

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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H6Gb1-CREB(1-280) C90A, T172C, T259C

Plasmid

#220234

Purpose

bacterial expression of CREB1 domains

Depositor

Peter Wright

Publication

Martinez-Yamout et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

CREB1 (Creb1 Rat)

Tags

His6-GB1-tev

Expression

Bacterial

Mutation

C90A, T172C, T259C

Promoter

T7

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTcI38-PRM-wasabi

Plasmid

#195554

Purpose

Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

Depositor

Mikhail Shapiro

Publication

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
TcI38

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T, M1V, L65S, K68R, F115L, D126G, D188G

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(T59/T61A)(HA)

Plasmid

#195025

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61A mutation

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)

Use

Gateway Cloning

Tags

3xHA

Mutation

T59/T61A

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-Creb5(T59/T61D)(HA)

Plasmid

#195026

Purpose

Gateway vector containing HA-tagged Creb5 with T59/T61D mutation

Depositor

Andrew Lassar, Cheng-Hai Zhang

Publication

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)

Use

Gateway Cloning

Tags

3xHA

Mutation

T59/T61D

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p11d-RPA70/14 (MSW#134)

Plasmid

#208076

Purpose

Expression of human Replication Protein A 70 and 14-kDa subunits in E. coli with 14 having a His-tag

Depositor

Marc Wold

Publication

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Insert

RPA1, RPA3 (RPA1, RPA3 Human)

Expression

Bacterial

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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