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pDEST-CMV 3xFLAG-GABARAPL2-GFP

Plasmid

#123113

Purpose

Expresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-GAlphasS-RLuc8

Plasmid

#140980

Purpose

Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GAlphasS-RLuc8

Use

Luciferase

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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GST scFv [N100/13]

Plasmid

#190521

Purpose

Mammalian Expression of GST scFV. Derived from hybridoma N100/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GST (Schistosoma japonicum) recombinant scFV

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Beta3

Plasmid

#140988

Purpose

Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GBeta3 (GNB3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pdest N-FLAG EGFP puro

Plasmid

#223056

Purpose

EGFP gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

EGFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1

Plasmid

#175049

Purpose

Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

Plasmid

#119946

Purpose

Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Renilla luciferase

Tags

24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

Expression

Bacterial and Mammalian

Promoter

TetCMV

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44s (fl)

Plasmid

#137812

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44s (fl) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

full length, I186T and I253V on CD44

Promoter

PGK

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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EGFR D770-N771 insNPG

Plasmid

#11016

Purpose

Retroviral construct for expressing human EGFR with D770-N771 insNPG mutation in human cells

Depositor

Matthew Meyerson

Article

Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

Insert

EGFR D770-N771 insNPG (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

D770-N771 insNPG

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pMH0001

Plasmid

#85969

Purpose

UCOE-SFFV-dCas9-BFP-KRAB

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Insert

dCas9

Use

Lentiviral

Tags

BFP-KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

SFFV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pST_140_LVL2 cam

Plasmid

#179334

Purpose

NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Mutation

Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pS6-hα2M-H6

Plasmid

#128495

Purpose

Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (G27-S1468) (A2M Human)

Tags

His tag (6x)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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PC1575

Plasmid

#232088

Purpose

Mammalian expression plasmid for ubiquibody (uAb) cloning backbone. Includes an Esp3I restriction site directly upstream of GSGSG linker and CHIPΔTPR CDS and a C-terminal IRES-mCherry cassette.

Depositor

Pranam Chatterjee

Article

Bhat et al Sci Adv. 2025 Jan 24;11(4):eadr8638. doi

Insert

uAb with cloning Esp3I restriction site

Tags

IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pFRT-DHX36iso1E335A

Plasmid

#159587

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

E335A

Promoter

CMV

Available Since

May 29, 2021

Availability

Academic Institutions and Nonprofits only

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pKT-CNP-inact

Plasmid

#211804

Purpose

Expresses inactive 2',3'-cyclic nucleotide phosphodiesterase catalytic domain (catalytic residues mutated)

Depositor

Emily Weinert

Article

Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

Insert

2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)

Expression

Bacterial

Mutation

H73L H153L

Promoter

tet

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV GFP-GABARAPL1 G116

Plasmid

#123119

Purpose

Expresses EGFP-GABARAPL1 G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma8-GFP2

Plasmid

#140990

Purpose

Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma8-GFP2 (GNG8 Human)

Tags

GFP2 and GSAG linker

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Neo_CAG-Cas9

Plasmid

#86698

Purpose

Cas9 expression vector for targeting to the AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

pCag

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_GR-RE_MLPmin_BC1094-luc2

Plasmid

#227114

Purpose

Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene

Use

AAV

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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