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We narrowed to 62,227 results for: cloning

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Showing: 9841 - 9860 of 62227 results

  5. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein i…
    Available Since
    Oct. 8, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    68709-plasmid-map-sequence-id-125636

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  6. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  7. pOliSpec5'

    Plasmid
    #228551
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid
    Depositor
    Jeferson Gross
    Insert
    Truncated 5' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228551-plasmid-map-sequence-id-454860

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  8. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

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  9. pDIV068

    Plasmid
    #247961
    Purpose
    Cloning vector containing A-PgpdA-PacI-Nt.BbvcI cassette-TtrpC-B sequences compatible with the DIVERSIFY gene landing platform
    Depositor
    Uffe Mortensen
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    247961-plasmid-map-sequence-id-501715

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  10. plix403-V5-N-mEYFP

    Plasmid
    #223955
    Purpose
    Gateway cloning of genes of interest with an N-terminal V5-mEYFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    V5-mEYFP
    Available Since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223955-plasmid-map-sequence-id-450839

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  13. pCWX-R4DESTR2-PGK-Tk:Sh

    Plasmid
    #114321
    Purpose
    Lentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Zeocin/Bleomycin resistance and Acyclovir conditional ablation
    Depositor
    Patrick Salmon
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114321-plasmid-map-sequence-id-219916

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  18. pJRA109

    Plasmid
    #209342
    Purpose
    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mTagBFP2 fusion protein.
    Depositor
    Lucia Strader
    Article
    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    209342-plasmid-map-sequence-id-420463

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Showing: 9841 - 9860 of 62227 results