We narrowed to 15,768 results for: LEA

1. pCoofy52

   Plasmid

   #122006

   Purpose

   E.coli vector for parallel SLIC cloning containing N-terminal His6-3xAvi with PreScission Cleavage site

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   His6-3xAvi

   Expression

   Bacterial

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAT10

   Plasmid

   #112591

   Purpose

   Used for expression of protein in E. coli as Thrombin cleavable N-termina 6His-tag-MBP fusions. Like pMAT9, but without the second BamHI site in the polylinker

   Depositor

   Marko Hyvönen, Johan Peränen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Tags

   6His-MBP

   Expression

   Bacterial

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. phSOD1-E100G-N’His

   Plasmid

   #117706

   Purpose

   mutation on stability/solubility of SOD1

   Depositor

   Elizabeth Fisher

   Insert

   SOD1 (SOD1 Human)
   

   Tags

   His tag with Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   E100G

   Promoter

   T7

   Available Since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pST50Trc4-FLAGDHFR

   Plasmid

   #63992

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   FLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST50Trc3-HISDHFR

   Plasmid

   #63971

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST50Trc2-FLAGDHFR

   Plasmid

   #63956

   Purpose

   Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Tags

   FLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. mOrange2-PCNA-19-SV40NLS-4

   Plasmid

   #57971

   Purpose

   Localization: Nucleus, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   mOrange2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pAAV.CAGFLEX.(mem).iGlucoSnFR2.mRuby3

   Plasmid

   #244082

   Purpose

   Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   iGlucoSnFR2.mRuby3
   

   Use

   AAV

   Tags

   IgG kappa leader and mRuby3

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.CAG.(mem).iGlucoSnFR2.mRuby3

   Plasmid

   #244075

   Purpose

   Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   iGlucoSnFR2.mRuby3
   

   Use

   AAV

   Tags

   IgG kappa leader and mRuby3

   Available Since

   Oct. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pI-SceI

    Plasmid

    #243802

    Purpose

    Contains the ORF for the homing endonuclease I-SceI under the control of the strong, constitutive yeast promoter PTDH3.

    Depositor

    Sai Reddy

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.hSynapFLEX.(mem).iGlucoSnFR2.mIRFP670nano3

    Plasmid

    #244102

    Purpose

    Plasma membrane targeted expression of green glucose sensor with non-responsive mIRFP

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    iGlucoSnFR2.mIRFP670nano3
    

    Use

    AAV

    Tags

    IgG kappa leader and mIRFP670nano3

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.hSynapFLEX.(mem).iGlucoSnFR2.mRuby3

    Plasmid

    #244101

    Purpose

    Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    iGlucoSnFR2.mRuby3
    

    Use

    AAV

    Tags

    IgG kappa leader and mRuby3

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.GFAP.(mem).iGlucoSnFR2.mRuby3

    Plasmid

    #244089

    Purpose

    Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Insert

    iGlucoSnFR2.mRuby3
    

    Use

    AAV

    Tags

    IgG kappa leader and mRuby3

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMB28

    Plasmid

    #223011

    Purpose

    Cas12a and Csy4 with 5' and 3' targets (I) for Nicotiana leaf assay

    Depositor

    Martin Parniske

    Article

    Bircheneder et al Plant J. 2024 Sep;119(5):2564-2577. doi:

    Insert

    Cas12a and Csy4 with 5' and 3' targets (I)
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDest17_Gcn4 WT 30mer

    Plasmid

    #231888

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 WT 30mer

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 30mer
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDest17_DBD

    Plasmid

    #231886

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 DBD

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 DBD
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDest17_Gcn4 WW+

    Plasmid

    #231885

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 WW+

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 WW+
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    E109N, S117D, T121W, N126W, T132W

    Promoter

    T7

    Available Since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDest17_Gcn4 Swap1

    Plasmid

    #231879

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 Swap1

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 Swap1
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDest17_Gcn4 R3

    Plasmid

    #231874

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 R3

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 R3
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    D103R, D133R, K143R

    Promoter

    T7

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDest17_Gcn4 P+

    Plasmid

    #231872

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 P+

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 P+
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    S122P, T131F, A141P, E143D

    Promoter

    T7

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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