We narrowed to 17,523 results for: emb

Showing: 9901 - 9920 of 17523 results

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HES7-NLuc-2A-tdTomato

Plasmid

#130932

Purpose

Donor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7 (HES7 Human)

Tags

NanoLuc and tdTomato

Expression

Mammalian

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHRSin-IRES2-EmGFP_HPC-4 Ab heavy-6xHIS

Plasmid

#187358

Purpose

Lentiviral expression of HPC-4 antibody heavy chain, and 6xHIS tag

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

HPC-4 antibody heavy chain, and 6xHIS tag

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry-pBirA-HA

Plasmid

#154458

Purpose

expresses mCherry-pBirA-HA/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry-pBirA-HA

Expression

Mammalian

Promoter

CMV-F, T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98H-pRc/CMV

Plasmid

#22044

Depositor

William Kaelin

Article

Ohh et al Mol Cell. 1998 Jun . 1(7):959-68.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant, converting aa 98 from Tyrosine to H…

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pEYFP-hDF-C2A

Plasmid

#72582

Purpose

human dysferlin C2A domain tagged with YFP

Depositor

Renzhi Han

Article

Xu et al PLoS One. 2011;6(11):e27884. doi: 10.137

Insert

dysferlin C2A domain (DYSF Human)

Tags

EYFP

Expression

Mammalian

Mutation

C2A domain

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Ki-67(RASA)-mCherry_IRESpuro2

Plasmid

#182921

Purpose

Expresses EGFP-Ki-67(RASA)-mCherry. Ki67 contains RASA mutation.

Depositor

Daniel Gerlich

Article

Cuylen-Haering et al Nature. 2020 Nov;587(7833):285-290. doi:

Insert

MKI67 (MKI67 Human)

Tags

EGFP and mCherry

Expression

Bacterial and Mammalian

Mutation

RASA mutation

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-neo-mApg5

Plasmid

#22956

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
pMx/ATP11C-HA

Plasmid

#209237

Purpose

Mammalian expression of ATP11C

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

Insert

ATP11C (ATP11C Human)

Use

Retroviral

Tags

HA

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 TIM8,13

Plasmid

#170282

Purpose

Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

Depositor

Paul Schanda

Article

Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

Insert

yeast Tim8 and Tim13 (TIM8 Budding Yeast)

Tags

TEV-cleavable N-terminal His tag on Tim13 (on bac…

Expression

Bacterial

Available Since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Tras heavy chain-GSG-SpyTag003

Plasmid

#216292

Purpose

Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

Tras heavy chain-GSG-SpyTag003

Use

Affinity Reagent/ Antibody

Tags

His6 tag, Signal peptide, and SpyTag003

Expression

Mammalian

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAM5082

Plasmid

#85121

Purpose

PkaiB-kaiC-ecfp expressed from NSII-Gm vector constructed by seamless assembly. Fusion expressed as full length fusion protein at WT levels but is 5hr long period. Used only for co-localization exp

Depositor

Susan Golden

Article

Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

Insert

PkaiB-KaiC-ECFP

Tags

ECFP

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-EEA1

Plasmid

#222419

Purpose

To express an early endosome "anchor" for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

EEA1 (EEA1 Human)

Tags

FRB-mCherry

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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AA025

Plasmid

#216005

Purpose

Fragmid fragment: (Cas protein) NG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9-NG_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-YOD1

Plasmid

#85663

Purpose

Expression of human YOD1 with N-terminal mCherry tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TREK-2

Plasmid

#224769

Purpose

In vitro transcription for Oocyte expression of 1 TREK-2

Depositor

Dan Minor

Article

Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

Insert

Potassium channel subfamily member 10 (Kcnk10 Mouse)

Use

In vitro transcription for oocyte expression

Promoter

T7

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AA299

Plasmid

#216029

Purpose

Fragmid fragment: (Cas protein) NNGG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9_v1.1 [Slug]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA296

Plasmid

#216028

Purpose

Fragmid fragment: (Cas protein) NNNNCC PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Nme2Cas9_v1.1 [Nme2]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA180

Plasmid

#216023

Purpose

Fragmid fragment: (Cas protein) NGRR(T) PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9_v1.1 [Sa]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA301

Plasmid

#216030

Purpose

Fragmid fragment: (Cas protein) NNGG PAM

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9_v1.1 [Sauri]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA027

Plasmid

#216007

Purpose

Fragmid fragment: (Cas protein) nearly PAM-less

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Cas9-SpRY_v1.1 [Sp]

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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