Easy Modular Integrative fuSion-ready Expression (Easy-MISE) Toolkit for Fast Engineering of Heterologous Productions in Saccharomyces cerevisiae.
Maestroni L, Butti P, Milanesi R, Pagliari S, Campone L, Serra I, Branduardi P
ACS Synth Biol. 2023 Apr 14. doi: 10.1021/acssynbio.3c00015. PubMed Article
Maestroni L, Butti P, Milanesi R, Pagliari S, Campone L, Serra I, Branduardi P
ACS Synth Biol. 2023 Apr 14. doi: 10.1021/acssynbio.3c00015. PubMed Article
These materials are included in the Easy-MISE Toolkit.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196336 | pGA-blue | Easy-MISE toolkit Level 0 destination plasmid for parts cloning with Esp3I-based Golden Gate Assembly; allowing fast postitive clones test with AmilCP chromoprotein-based blue/white screening |
| 196337 | pGA-red-maxi | Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening |
| 196338 | pGA-red-mini | Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening |
| 198641 | pEM.P01L | Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with EF protruding ends |
| 198642 | pEM.P01R | Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with FG protruding ends |
| 198643 | pEM.P02L | Easy-MISE toolkit pEM-plasmid containing ENO2 promoter with EF protruding ends |
| 198644 | pEM.P02R | Easy-MISE toolkit pEM-plasmid containing ENO2 promoter with FG protruding ends |
| 198645 | pEM.P03L | Easy-MISE toolkit pEM-plasmid containing PGK1 promoter with EF protruding ends |
| 198646 | pEM.P03R | Easy-MISE toolkit pEM-plasmid containing PGK1 promoter with FG protruding ends |
| 198647 | pEM.P04L | Easy-MISE toolkit pEM-plasmid containing TPI1 promoter with EF protruding ends |
| 198648 | pEM.P04R | Easy-MISE toolkit pEM-plasmid containing TPI1 promoter with FG protruding ends |
| 198649 | pEM.P05L | Easy-MISE toolkit pEM-plasmid containing CYC1 promoter with EF protruding ends |
| 198650 | pEM.P05R | Easy-MISE toolkit pEM-plasmid containing CYC1 promoter with FG protruding ends |
| 198651 | pEM.P06L | Easy-MISE toolkit pEM-plasmid containing PDA1 promoter with EF protruding ends |
| 198652 | pEM.P06R | Easy-MISE toolkit pEM-plasmid containing PDA1 promoter with FG protruding ends |
| 198653 | pEM.T01L | Easy-MISE toolkit pEM-plasmid containing ADH1 terminator with BC protruding ends |
| 198654 | pEM.T02R | Easy-MISE toolkit pEM-plasmid containing CYC1 terminator with IL protruding ends |
| 198655 | pEM.A01L | Easy-MISE toolkit pEM-plasmid containing a spacer sequence with CD protruding ends |
| 198656 | pEM.A01R | Easy-MISE toolkit pEM-plasmid containing a spacer sequence with HI protruding ends |
| 198657 | pEM.A02L | Easy-MISE toolkit pEM-plasmid containing a spacer sequence with BF protruding ends |
| 198658 | pEM.A02R | Easy-MISE toolkit pEM-plasmid containing a spacer sequence with FL protruding ends |
| 198659 | pEM.F01L | Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with CD protruding ends |
| 198660 | pEM.F01R | Easy-MISE toolkit pEM-plasmid containing GFP coding sequence with HI protruding ends |
| 198661 | pEM.F02L | Easy-MISE toolkit pEM-plasmid containing GFP coding sequence preceded by a linker sequence with CD protruding ends |
| 198662 | pEM.F02R | Easy-MISE toolkit pEM-plasmid containing GFP coding sequence preceded by a linker sequence with HI protruding ends |
| 198663 | pEM.F03L | Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with CD protruding ends |
| 198664 | pEM.F03R | Easy-MISE toolkit pEM-plasmid containing mCherry coding sequence with HI protruding ends |
| 198665 | pEM.H01L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198666 | pEM.H01R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198667 | pEM.H02L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198668 | pEM.H02R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198669 | pEM.H03L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198670 | pEM.H03R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198671 | pEM.H04L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198672 | pEM.H04R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198673 | pEM.H05L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198674 | pEM.H05R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198675 | pEM.H06L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198676 | pEM.H06R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198677 | pEM.H07L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198678 | pEM.H07R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198679 | pEM.H08L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198680 | pEM.H08R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198681 | pEM.H09R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198682 | pEM.H09L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198683 | pEM.H10L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198684 | pEM.H10R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198685 | pEM.H11L | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |
| 198686 | pEM.H11R | An Easy Modular Integrative fuSion-ready Expression toolkit (Easy-MISE toolkit) for fast engineering of heterologous productions in Saccharomyces cerevisiae |