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pHR_Gal4UAS_Blinatumomab_PGK_mCherry

Plasmid

#85437

Purpose

[Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

Blinatumomab

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher002

Plasmid

#102827

Purpose

Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

Depositor

Mark Howarth

Article

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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mEGFP-4xbiRhoBAST

Plasmid

#196350

Purpose

Expression of mEGFP mRNA with 4xbiRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-4xbiRhoBAST

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-oG

Plasmid

#74288

Purpose

To express oG (optimized Glycoprotein) under CAG promoter

Depositor

Edward Callaway

Article

Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

Insert

oG

Expression

Mammalian

Mutation

chimeric glycoprotein

Promoter

CAG

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pS448∙CsR

Plasmid

#122096

Purpose

CRISPR/Cas9 counterselection in Gram-negative bacteria

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Inserts

Cas9
sgRNA

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pem7 and Pm

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pPhlF-BCD20-sfGFP-ssrA P64U23C39T13 v1-1 (462)

Plasmid

#70741

Purpose

1 color strong reporter used in study

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

sfGFP-ssrA

Available Since

Feb. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRRL-TetO-Pax7-IRES-EGFP

Plasmid

#109372

Purpose

Expresses Dox-inducible Pax7 together with a GFP reporter

Depositor

Nenad Bursac

Article

Rao et al Nat Commun. 2018 Jan 9;9(1):126. doi: 10

Insert

Pax7

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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XBP-1 1: pFLAG.XBP1u.CMV2

Plasmid

#21832

Depositor

David Ron

Article

Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

Insert

XBP-1 unprocessed (Xbp1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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Synapsin-cyto-iATPSnFR1.0

Plasmid

#102556

Purpose

expresses cytoplasmic ATP sensor in neurons

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

AAV

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMOD_C0000

Plasmid

#91081

Purpose

Module C, Promoter: none, Gene: none (BaeI site for GT donor cloning), Terminator: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Unspecified

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEL685

Plasmid

#205592

Purpose

NEPE for prime editing in rice

Depositor

Yong Zhang

Article

Zhong et al Plant Commun. 2024 May 14:100976. doi: 1

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

ZmUbi

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor14-N7HELIX-KanR

Plasmid

#190663

Purpose

Kanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Promoter

NA

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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RUBYm2

Plasmid

#232175

Purpose

Introduce a C-to-T mutation to generate an early stop codon (Q139 to stop), thereby interrupting RUBY expression

Depositor

Yiping Qi

Article

Sexton et al Plant Physiol. 2025 Jul 3;198(3):kiaf265

Insert

RUBY mutant2 and CaMV 35S promoter

Expression

Plant

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-P2A-BFP

Plasmid

#149447

Purpose

Expresses Cas9 and BFP in a lentiviral vector.

Depositor

Jonathan Chernoff

Article

Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

Insert

P2A-BFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pDD104 (Peft-3::Cre)

Plasmid

#47551

Purpose

Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Insert

Cre Recombinase

Use

Cre/Lox

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGY214

Plasmid

#239805

Purpose

Express EGFP and mRFP1 simultaneously in filamentous fungi

Depositor

Kyle Pomraning

Article

Guoliang et al ACS Synthetic Biology Article ASAP

Inserts

EGFP
mRFP1

Use

Fungal expression

Promoter

Tef1IN

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pDonor14-ShHELIX-KanR (CJT70)

Plasmid

#181793

Purpose

pDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-Camk2-NES-Twitch-GR

Plasmid

#173044

Purpose

TnC-based calcium sensor with green-red FRET pair ratiometric readout

Depositor

Yiyang Gong

Article

Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

Has Service

AAV2

Insert

NES-Twitch-GR

Use

AAV

Expression

Mammalian

Promoter

CamK2

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ143

Plasmid

#69295

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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