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pAV-U6+27-Linear-1_PolyU(4)_Tornado-Corn

Plasmid

#159482

Purpose

Tests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Publication

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(4)

Expression

Mammalian

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(5)_Tornado-Corn

Plasmid

#159483

Purpose

Tests for the impact of 5 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Publication

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(5)

Expression

Mammalian

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(6)_Tornado-Corn

Plasmid

#159484

Purpose

Tests for the impact of 6 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Publication

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(6)

Expression

Mammalian

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(7)_Tornado-Corn

Plasmid

#159485

Purpose

Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Publication

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(7)

Expression

Mammalian

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(8)_Tornado-Corn

Plasmid

#159486

Purpose

Tests for the impact of 8 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Publication

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(8)

Expression

Mammalian

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-ydfHp-GFPuv

Plasmid

#139079

Purpose

Low phosphate inducible promoter ydfHp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

ydfHp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-phnCp-GFPuv

Plasmid

#139080

Purpose

Low phosphate inducible promoter phnCp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phnCp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-tktBp-GFPuv

Plasmid

#139081

Purpose

Low phosphate inducible promoter tktBp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

tktBp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-asrp-GFPuv

Plasmid

#139082

Purpose

Low phosphate inducible promoter asrp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

asrp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-yegHp-GFPuv

Plasmid

#139084

Purpose

Low phosphate inducible promoter yegHp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

yegHp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-ytfKp-GFPuv

Plasmid

#139085

Purpose

Low phosphate inducible promoter ytfKp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

ytfKp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-amnp-GFPuv

Plasmid

#139070

Purpose

Low phosphate inducible promoter amnp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

amnp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-mipAp-GFPuv

Plasmid

#139071

Purpose

Low phosphate inducible promoter mipAp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

mipAp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoEp-GFPuv

Plasmid

#139074

Purpose

Low phosphate inducible promoter phoEp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoEp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoHp1-GFPuv

Plasmid

#139075

Purpose

Low phosphate inducible promoter phoHp1 for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoHp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-phoUp-GFPuv

Plasmid

#139076

Purpose

Low phosphate inducible promoter phoUp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

phoUp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHCKan-pstSp-GFPuv

Plasmid

#139077

Purpose

Low phosphate inducible promoter pstSp for GFP expression

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

Insert

pstSp-GFPuv

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRepair-mTq2-CTNNB1

Plasmid

#153433

Purpose

Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2

Depositor

Renee van Amerongen

Publication

de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

Insert

CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)

Use

CRISPR; Hdr donor template

Tags

mTurquoise2

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSP569

Plasmid

#139499

Purpose

Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

Depositor

Serge Pelet

Publication

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xPP7sl STL1 100 - 600

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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Y365A or A365Y inducible rescue

Plasmid

#131323

Purpose

gRNA to generate i-WT-r or i-MT-r systems

Depositor

Danny Reinberg

Publication

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

Y365A or A365Y inducible rescue

Use

CRISPR

Expression

Mammalian

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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