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We narrowed to 227 results for: trypanosoma

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Showing: 81 - 100 of 227 results

  3. pHJ17

    Plasmid
    #48356
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48356-plasmid-map-sequence-id-70042

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  4. pHJ22

    Plasmid
    #48358
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Neomycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-NEO-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48358-plasmid-map-sequence-id-70047

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  6. pHJ18

    Plasmid
    #48357
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48357-plasmid-map-sequence-id-70046

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  7. pHJ41

    Plasmid
    #48360
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48360-plasmid-map-sequence-id-70051

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  8. pHJ42

    Plasmid
    #48359
    Purpose
    Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Bleomycin/Gancyclovir selectable
    Depositor
    George Cross
    Article
    Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1
    Insert
    Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-BLE-Ty1-TK-loxP-3' ALD UTR
    Use
    Cre/Lox; Knockout in t. brucei
    Available Since
    Jan. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    48359-plasmid-map-sequence-id-70049

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  14. p221- purVSG117UTR

    Plasmid
    #59732
    Purpose
    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.
    Depositor
    Gloria Rudenko
    Article
    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:
    Insert
    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    Use
    For homologous recombination into vsg221 expressi…
    Available Since
    Aug. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    59732-plasmid-map-sequence-id-100249

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  15. SpIMPDH

    Plasmid
    #206980
    Purpose
    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.
    Depositor
    Vern Schramm
    Article
    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210
    Insert
    SpIMPDH (guaB Streptococcus pyogenes)
    Tags
    His tag (with TEV cleavage site)
    Expression
    Bacterial
    Available Since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    206980-plasmid-map-sequence-id-410397

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  16. pLEW100v5-BSD-FLAG-La-Cas9

    Plasmid
    #245102
    Purpose
    Inserts tetracycline-inducible codon optimized, nuclceus targeted FLAG-Cas9 into T. brucei cells at the rDNA spacer
    Depositor
    Monica Mugnier
    Article
    Smith et al Nature (2026)
    Insert
    3xFLAG-La-Cas9
    Use
    CRISPR
    Tags
    3xFLAG-La
    Mutation
    codon optimized for T. brucei
    Available Since
    April 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    245102-plasmid-map-sequence-id-489639

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  17. pLEW100v5-trunc

    Plasmid
    #33346
    Depositor
    George Cross
    Insert
    Luciferase truncated to allow cytoplasmic instead of peroxisomal (glycosomal) expression
    Use
    Trypanosoma brucei expression vector
    Mutation
    21bp of wt luciferase deleted
    Available Since
    Dec. 1, 2011
    Availability
    Academic Institutions and Nonprofits only
    33346-plasmid-map-sequence-id-39752

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  18. pRP-iSL(MCS1+2)

    Plasmid
    #69245
    Purpose
    Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a random RRNA spacer locus in T. brucei.
    Depositor
    David Horn
    Type
    Empty backbone
    Use
    Expression in trypanosoma brucei
    Promoter
    RRNA promoter
    Available Since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 81 - 100 of 227 results