We narrowed to 243 results for: trypanosoma
-
Plasmid#210215PurposeIntegrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.DepositorInsert162xLacO repeats derived from: PMID: 12864855.
Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-blast
Plasmid#210221PurposeIntegrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.DepositorInsert50xLacO repeats derived from: PMID: 12864855.
Available SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLew100_myc_BirA*
Plasmid#41716DepositorTypeEmpty backboneUseT. brucei expressionPromoterPARPAvailable SinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
TbSIR2RP1
Plasmid#67242PurposeDetermine its activity and specificityDepositorInsertTbSIR2RP1
TagsMBP, TEV, Thrombin, HIS, and noneExpressionBacterialPromoterp-lacAvailable SinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLew100::NLS-ISceI-HA
Plasmid#21299DepositorAvailable SinceDec. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBES1-LacOx50-mCh-bla
Plasmid#210260PurposeIntegrates 50xLacO repeats and an mCherry gene upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
mCherry
Available SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Luc-Neo
Plasmid#48336PurposeTrasgenically expresses firefly luciferase in T. cruzi with neomycin selectionDepositorInsertfirely luciferase
UseTrypanasoma cruzi expressionPromoterT. cruzi rRNAAvailable SinceJuly 20, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC_sgRNA
Plasmid#68710PurposeContains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyAvailable SinceSept. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSY45
Plasmid#48355PurposeTargets TbMCM-BP for Cre-lox mediated knockout in T. bruceiDepositorInsertloxP-SAS-HYG-Ty1-TK-loxP flanked by TbMCM-BP-targeting homologies
UseCre/Lox; Knockout in t. bruceiAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMOTag-M9-4H
Plasmid#106379PurposeCan be used as a PCR template to amplify the M9-HA point mutant of glmS fragment for gene specific tagging that only allows tagging but not regulation. Acts as a control for glmS based gene regulationDepositorInsertGlmS with M9 mutation
Available SinceMarch 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDS66
Plasmid#48364Purpose3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsert3XHA-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiAvailable SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDS67
Plasmid#48365Purpose3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsert3XMYC-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiAvailable SinceOct. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDS68
Plasmid#48366PurposeGFP-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsertGFP-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDS69
Plasmid#48367PurposeFLAG-intergenic region of TUB-HYG (modified from PMID 16269191)DepositorInsertFLAG-intergenic region of TUB-HYG (modified from PMID 16269191)
UseCre/Lox; Knockout in t. bruceiAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx120-20xMS2-Hyg
Plasmid#210220PurposeIntegrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
Available SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHJ17
Plasmid#48356PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin/Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHJ18
Plasmid#48357PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHJ41
Plasmid#48360PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Blasticidin/Gancyclovir selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-BSD-Ty1-TK-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHJ32
Plasmid#48361PurposeTargets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Hygromycin selectableDepositorInsertPartial beta-TUB followed by 5' ALD UTR-loxP-SAS-HYG-loxP-3' ALD UTR
UseCre/Lox; Knockout in t. bruceiAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only